The Experimental Research About Same Disease With Different Syndromes Of Alzheimer's

Objective: This animal testing studies two models of diseases combined syndromes, to take disease(Alzheimer's Disease, simply "AD" for short) and the constituent of syndromes(yang deficiency of kidney, turbid phlegm blocking clear orifice)as object of study. we can test the change of indexes about six main pathogenesis(Abnormal excessive phosphorylation of Tau protein, Amyloid precursor protein metabolic disorders, Cholinergic system, Nerve cell apoptosis, Ubiquitin-Proteasome pathway, Immune inflammation response) of AD, such as Tau protein, A?, Bax, Bcl-2, Ub, E1, P65, Ach, Ach E, on the condition of foregoing pathogenesis. We will explore biological intension and interposing pathogenesis of recipes about two typical syndromes of AD and clarify the scientific intention of the universality and specificity about the biological pathogenesis of AD in same disease with the different syndromes and the prescription-syndrome.Methods: Thirteen SAMR1 mice(Six months of the little mouse) were allocated in Control Group(simply "Normal Group" for short), thirty nine SAMP8 mice(Six months of the little mouse) were randomly divided into AD model group(simply "Model Group" for short), AD of Kidney Yang Deficiency Syndrome group(simply "Kidney Empty Group" for short), AD of Phlegm Blocking Orifices Syndrome Group(simply "Phlegm Turbidity Group” for short),with 13 mice in each of these. In order to get the mice with AD of Kidney Yang Deficiency Syndrome, we gave each rat of Kidney Yang Deficiency Syndrome group an injection of hydrocortisone. And we fed high fat diet to the mice of Phlegm Blocking Orifices Syndrome group for 6 weeks to get the mice with AD of Phlegm Blocking Orifices Syndrome. After the model was successfully copied, we collected the specimen, and then observed the generality and specificity of above two types of syndrome in micro aspect through light microscope, electron microscope, biochemical index, etc.Results:1.The morphological results The results of optical microscope observation of liver and kidney and electron microscope observation of hippocampus showed that, liver and kidney and hippocampus ultrastructure of mice in Normal Group had no obvious abnormal. And mild damages of them lied in mice in Model Group. And the damages of them in Phlegm Turbidity Group were more serious than Model Group, while the damages of Kidney Empty Group were the most serious. 2.Biochemical test results 2.1 Model Group compared with the Normal Group:The index content, such as Tau protein, A?, Bax of mice in Two groups had significant difference(P<0.05), the content of Tau protein, A? and Bax in Model Group were higher than Normal Group. These two groups of mice showed no significant difference in the content of Ach, Ach E, Bcl-2, Ub, E1, P65. 2.2 Kidney Yang Deficiency Syndrome Group compared with the Normal Group:ACTH and CORT content in Two groups of mice were significantly different(P<0.05), the content of ACTH and CORT in Kidney Yang Deficiency Syndrome Group were lower than Normal Group. 2.3 Phlegm Blocking Orifices Syndrome Group compared with Normal group: TG content in blood have significant difference in two groups(P<0.05), the content of TG in Phlegm Blocking Orifices Syndrome Group were higher than Normal group,and is 1.56 times that of in Normal Group. 2.4 Kidney Yang Deficiency Syndrome Group compared with Phlegm Blocking Orifices Syndrome Group: the indexes content in two groups of mice had no significant difference.The content of Tau protein, A?, Bax in Kidney Yang Deficiency Syndrome Group were higher than Phlegm Blocking Orifices Syndrome Group, Bcl-2 were lower than group Phlegm Blocking Orifices Syndrome,and they had obvious regularity(P<0.05). Ach, Ach E, Ub, E1, P65 content of rat had no obvious regularity.Conclusion: There are obvious anomaly between the Kidney Yang deficiency Syndrome AD and Phlegm Blocking Orifices Syndrome AD, on the Tau protein phosphorylation, Amyloid precursor protein metabolic, and Nerve cell apoptosis of six major mechanisms. In comparison, the anomaly and damage in kidney Yang Deficiency Syndrome is more serious on the three mechanisms.