Research On Effect Mechanisms About Correspondence Of Prescription And Syndrome Of Disease Combination About AD Of Kidney Yang Deficiency Syndrome And Phlegm Obstruction In The Orifices Syndrome

Objective: Through to establish ani Pl models combined disease and dementia,in the method of the corresponding(golden Kidney Yang Deficiency Syndrome to give kidney pellet preparation,Phlegm Obstruction in the Orifices Syndrome given wendan tang agents),and explore the TLo typical AD syndrome prescription intervention effect mechanism,to explore the AD different syndrome,party card corresponding prescriptions in generality and specificity of pny in the pathogenesis and effect of the specific indicators.Methods: There are seventy-eight TLo SAM mice,including thirteen SAMR1 mice,sixty-five SAMP8 mice.Thirteen SAMR1 mice were allocated in Control Group,thirty nine SAMP8 mice were randomly divided into AD model group,AD kidney(SM)group,AD of Kidney Yang group,AD of Kidney Yang Deficiency Syndrome group,AD of Phlegm Blocking Orifices Syndrome Group,with 13 mice in each of these.R,P group according to the dose of 1 ml / 100 g,4 weeks with 0.9% saline lavage(W),continuous every day SL group mice celiac injection of hydrocortisone,according to the dose of 0.3 ml / 100 g,once a day,a total of 9 days;TL group high fat feed 6 w,high fat feed formula ratio for: basic feed 78.5%,egg yolk powder 10%,lard of l0 %,cholesterol(1%),the re Pining 0.5% of propylthiouracil.Through the above methods,respectively,set up AD Kidney Yang Deficiency Syndrome and Phlegm Obstruction in the Orifices Syndrome,combining phlegm turbidity and disease resistance model,and intragastric administration(give the corresponding kidney pills and wendan tang preparations intervention).From electron microscope slices and biochemical indexes of hippocampus and the cerebral cortox in mice,mice were sliced(kidney,liver)and from different aspects,such as blood index observation card corresponding internal mechanism.Results:1.Blood biochemical indicator detection results showed that: compared with the SA group,there is a downtrend of TG index in the TL group having a statistics significant difference(P < 0.05);Compared with the TM group,there is a rise of ACTH and CORT index in the TL group,and the difference is significant(P < 0.05).2.The observed results showed:There are different degrees of injury of hippocampus,liver,kidneys between the P group,SM group,SL group,TM group,TL group of mice,the dapge degree of liver from light to heavy,in order: P group,TL group,SL group,TM group,SM group;the dapge degree of kidney from light to heavy,in order:P group,TL group,TM group,SL group,SM group.3.Electron microscopy results showed that hippocampus of the P group,SM group,SL group,TM group,TL group in mice hippocampal ribosomes,mitochondria,the structure of the synapses,synaptic vesicles,rough endoplasmic reticulum are all had some degree of dapge.The degree of dapge is from light to weight: P group,TL group,SL group,TM group,SM group.4.Hippocampal cortex biochemical index test results showed that :compared with the R group,the rest of the five groups of mice in the hippocampus Ub and P65 all have increased,Compared with the SM group,the E1 index in SL group was trending down,compared with the TM group,the TL group E1 index was decreasing,the difference is not statistically significant;Tau protein,A ?,Bax,Ach E all have increased,The second SL group and the TL group compared with the model group with the model were declining;the Bcl-2,Ach have declined,The second SL group and the TL group were up in comparison with the model group.and the difference is significant(P< 0.05).Conclusion:1.In this experiment,pathological damages in Kidney Yang Deficiency Syndrome groups were more severe than those in the Phlegm Blocking Orifices Syndrome groups.Pathological damages may present different severity in different syndromes of the same disease.2.Through to party card accordingly Kidney golden pellet preparation were given through Kidney Yang Deficiency Syndrome,Phlegm Obstruction in the Orifices Syndromephlegm Obstruction in the Orifices Syndrome give wendan tang preparation,it can correct the abnorpi Tau protein,A ? and cell apoptosis.It indicates that prescription-syndrome correspondence Py have a multiple effects on dislease.3.When the party card accordingly,it can correct the bippocampus related biochemical indexes(Tau albumen,A?,Bax,Ach E),and it also can improve the disorder of abnorpi stresl(TG,ACTH,CORT)to a certain extent,improve the index of neuroendocrine system and blood lipid,the explanation is that the pathologic changes of the disease can be tailored to the pathological changes of the disease.explain party card corresponding in the treatment of diseases is comprehensive.4.This experiment has not yet found the difference in the mechanism of the different evidence types.