| Objective: microcirculation pathology is present in the heart and cerebrovascular diseases, diabetes, cancer, and many other high-risk disease, microcirculation and blood stasis of Chinese medicine are closely linked, give full play to advantages of Chinese medicine treatment microcirculation disease has important significance. This observation of the Gexia Zhuyu decoction its separate prescriptions on the efficacy and me chanism of the microcirculation of rat model for further study of the influence of Gexiazhuyu decoction provide a theoretical basis for clinical medication.Methods: polymer dextran intravenous injection method of rat model of microcirculation. 50 Wistar rats were randomly divided into five groups of 10 mice in each control group(referred to as "the normal group"), model group(referred to as "model group"), Gexiazhuyu decoction high dose group( referred to as "high-dose group"), Gexiazhuyu decoction low-dose group(referred to as "low dose"), blood demolition party group(referred to as "demolition party group"). Model group, high dose group, low dose group, party groups were split 10% dextran polymer tail vein injection at a dose of 10 ml / Kg, injected once every other day, continuous injection of 10 times. Tail vein injection every other day in the control group of 0.9% saline, at a dose of 10 ml / Kg. Administration group in modeling on the basis of oral administration, the amount of orally 10 ml / Kg, once a day orally, intragastric administration of ten times. Wherein Gexiazhuyu decoction high dose group at a dose of 7.5g / Kg, a dose of low dose group 11.5 g / Kg; Huoxue Fang group to split in angelica, peach kernel, safflower, Chuan Xiong, Yuan Hu decoction, a daily dose of 3g / Kg. Control group, model group was given in purified water. Oral gavage volume of 10 ml / Kg, every other day for 10 times. After the last administration, the animals were fasted the night, can not help but water, experimental testing of the first two days. 5% chloral hydrate anesthesia, celiac mesenteric microcirculation first observed(mainly detected velocity, flow regime, arteriovenous ratio, observe whether the change in blood stasis; secondly observe whether the bleeding, etc.), followed by intraperitoneal blood drawn, hemorrheology to detect activity of vascular endothelial factor(NO, ET, VEGF, ES content), etc., to explore microcirculation blood stasis internal mechanisms for change. And the liver, lungs and other organs and tissues of liver, lung histopathology is detected within key observation microcirculation stasis and other changes injury.Results: Gexiazhuyutang high and low dose group rats microcirculation has a good effect, and promoting blood circulation demolition party group as effective as whole-group.(1) lung, liver microcirculation injury treatment. First, the treatment of lung injury: give Gexiazhuyudecoction after high-dose group of rats between low quality bleeding, bleeding condition were significantly reduced, significantly reduced infiltration of inflammatory cells, reduce the degree of septal thickening, in particular to improve the effect of high-dose group Gexiazhuyudecoction lung microcirculation damage is more obvious, P <0.05, statistically significant results. The effect of improving the demolition party group is not as Gexiazhuyudecoction high and low dose group, P <0.05, statistically significant results. Second,the liver tissue injury treatment: Gexia Zhuyu Decoction high, uniform low dose group liver cells, no degeneration and necrosis, lobular structure is more clear, liver fiber recovery interval, blood stasis condition of the red blood cell aggregation has been relatively good improvement, especially high-dose treatment group was more obvious. Microcirculation activating blood stasis state party split liver group has also been improved, but the effect is not as high and low dose groups.(2) the effect of mesenteric microcirculation injury: Gexiazhuyudecoction high and low dose group of arterial and venous flow rate increased significantly, mainly as blood flow pattern lines flow, blood stasis state improved. Huoxue Fang demolition group, blood flow increased, P<0.05, statistically significant results, mesenteric microcirculation corresponding improvement, but not as good as the effect of improving Gexiazhuyudecoction administration group. Give Gex iazhuyudecoction after high-dose group and low dose group rat mesenteric artery thickening micro, venules thinner, DA / DV ratio becomes large, P <0.05, statistically significant results, the data results can be displayed mesenteric vascular wall damage improved, DA / DV ratio is close to the normal group, mesenteric microcirculation microcirculation in good condition, and the treatment effect Gexiazhuyudecoction high-dose group than the low dose group. Demolition Huoxue Fang Group mesenteric, vein pathological conditions also have a certain effect, but not as effective as treatment Gexiazhuyudecoction administration group, P <0.05, statistically significant results.(3) improve blood rheology indicators: Gexiazhuyudecoction high and low dose of whole blood high and low shear viscosity values, and plasma viscosity, whole blood high and low shear reduced viscosity, hematocrit, erythrocytes rigidity index, erythrocyte aggregation index have a significantly reduced red blood cell deformability index(TK) there has been increased, P <0.05, statistically significant results, the therapeutic effect of activating blood demolition party group is better Gexiazhuyudecoction administration group.(4) the efficacy of vasoactive factors: Gexiazhuyudecoction high and low dose NO content increased, ET content decreased, the difference was statistically significant(P <0.05), the results may indicate a pathological condition apparent stasis mitigation, but also get a better microcirculation improved, and the effect of improving Gexiazhuyudecoction high-dose group than the low dose group. Huoxue Fang demolition lung tissue NO content increased slightly, ET content increased significantly, the proportion of NO and ET in serious disorder, but the difference was not statistically significant. Huoxue Fang demolition plasma NO content increased, ET content has increased sharply, and the ET content was not statistically significant. Gexiazhuyudecoction high and low dose of VEGF decreased, ES content increased, the difference was statistically significant(P <0.05), results can be displayed in lung tissue damage repaired, lung microcirculation is improved, and the diaphragm Gexiazhuyu decoction efficacy of high-dose group than the low dose group. Demolition party group compared with model group, the demolition side of VEGF was significantly reduced, ES content increased, the difference was statistically significant(P <0.05), activating blood visible side dismantle the group have a therapeutic effect on lung damage, but the treatment effect far less Gexiazhuyudecoction high and low dose group. Rat plasma VEGF and ES content: As can be seen from the data in the table result, the normal group, model group, and Gexiazhuyudecoction low dose group, blood plasma VEGF demolition party content is extremely low, and the difference was not the result Statistical significance. Gexiazhuyudecoction high, reducing the blood of rats in the low dose group content ES and ES content is less than the high dose group and low dose group, the difference was statistically significant(P <0.05). Huoxue Fang demolition ES group content increases, the difference was statistically significant(P <0.05).Conclusion: Gexiazhuyudecoction high and low dose group rats microcirculation has a good effect, and promoting blood circulation demolition party group as effective as full parties Zhuyu decoction group and promoting blood circulation and cooling gas line drugs and hemostatic compatibility role is important. |
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