| Objective: Breast cancer is one of the most common malignant tumors in women, With the increasing pressure of women's life and work, the age of onset for the disease is becoming younger and younger, which has become one of the important diseases that threaten the health of women of all ages. At present, chemotherapy is still an important method for the treatment of breast cancer, in which the platinum compounds play an important role. The research and application of platinum compounds have been developed rapidly. A few thousand kinds of platinum compounds have been synthesized, but in clinical practice use only about 30 kinds of investment. As a classic anticancer drug, cisplatin has been widely used in clinical practice. But cisplatin has high drug resistance, neurotoxicity and renal toxicity, which limits its development to a certain extent. New platinum compounds, such as Pt(IV) complexes, have been synthesized in order to overcome the side effects of cisplatin. In this paper, the effects of different concentrations of Pt(IV) and cisplatin on the growth of MCF-7 breast cancer cell line in nude mice were observed.Method:20 BALB/Cnu female nude mice, aged from 4 to 6 weeks, MCF-7 single cell suspension were inoculated in nude mice left forelimb armpits on the second breast pad, MCF-7 nude mice model was established and randomly divided into 4 groups: control group(n = 5), high dose of Pt(IV) complexes group(n = 5), low dose of Pt(IV) complexes group(n = 5), cisplatin group(n = 5). Every Monday and Thursday, mice of the 4 groups were given intraperitoneal injection of drugs according to the regimen, general living conditions were recorded,the growth of the transplanted tumor in nude mice and the changes of body weight were dynamically observed, tumor volumes were measured and growth curve was drawn. The nude mice were killed after 3 weeks of treatment and the quality of tumor was weighed. Tumor inhibition rate was calculated and the tumor tissue platelet endothelial cell adhesion molecule(CD31) expressions were detected by immunohistochemistry. All the data was described with( x ± s),SPSS19.0 statistical software was used to deal with data, mean compared by test, with P < 0.05 for the difference was statistically significant.Result:(1)The weight of the tumor-burdened nude mice in the four groups was not significantly changed after the treatment. There were no significant differences in the effects between Pt(IV) complex and cisplatin on the diet and body weight of the nude mice.(2) The tumor volume of high dose Pt(IV) complex group and cisplatin group was smaller than that of the control group,respectively,and the differences were statistically significant(P < 0.001). However, there was no significant difference in the tumor volume between the low dose Pt(IV) complex group and the control group(P > 0.05). Both high dose Pt(IV) complex and cisplatin could significantly inhibit the growth of subcutaneous the transplanted tumor in nude mice.(3) The tumor suppressed rates of high dose Pt(IV) complex group and cisplatin group were 45.2% and 53.3%, respectively. The weight of transplantable tumors of the high dose Pt(IV) complex group was lower than that of the control group and the difference was statistically significant(P < 0.001). So was the cisplatin group. Both high dose of Pt(IV) complexes and cisplatin could inhibit tumor growth.(4) In the control group, the expression of CD31 was significantly higher than that of the high dose Pt(IV) complex group and the cisplatin group, and the differences were statistically significant(P < 0.001). Both high dose Pt(IV) complexes and the cisplatin could reduce the expression of CD31.Conclusion:By constructing the nude mice model with MCF-7 breast cancer cell line found that high dose Pt(IV) complex and cisplatin could inhibit tumor growth and low dose of Pt(IV) complex is ineffective. High dose of Pt(IV) complex and cisplatin could reduce the expression of CD31 in nude mice transplanted tumor tissue. |
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