The Effect Of Nine Common Antibiotics On The Release Of Endotoxin From Gram Negative Bacteria

When selecting antibiotic treatment of infections in the body of a conventional veterinary clinic, focused on considering the type of bacterial infection side effects, drug sensitivity and drug. When Gram-negative bacteria infections should be treated to eliminate bacteremia and endotoxemia. With the development of science and technology, the antimicrobial efficacy of the treatment of infectious diseases has been greatly improved. But the shock associated with the infection caused by endotoxemia, the incidence and mortality rates remain high. This test is commonly used in clinical veterinary selected three groups of 9 different types of antimicrobial agents are effects to both Gram-negative bacteria, using the kinetic turbidimetric endotoxin content within the filtrate to detect endotoxin level comparison, further explore the relationship between the mechanism of action of antibiotics, so that it can better guide clinical veterinary correct medication.Selected the common veterinary clinical GNB infection of E.coli and Salmonella, bacteria were exposed to Amoxicillin(AMO), Ceftriaxone(CRO), Cefotaxime(CTX), Kanamycin(KAN), Gentamycin(GM), Amikacin(AMK), Ciprofloxacin(CIP), Enrofloxacin(ENR), Ofloxacin(OFL) at 0.5MIC, 2MIC, 5MIC and 10 MIC. Samples(1m L)were obtained at 0h, 2h, 4h, 6h, 8h after bacteria exposing to antibiotics. LPS release was measured by Tachypleus amebocyte lysate Test(TAL Test). The culture without antibiotic acted as a control, broth without drug and bacteria was a blank tube.When E. coli and salmonella were treated with three types of antibiotics, endotoxin release is ?-lactams> quinolones> aminoglycosides. However, where the other two drugs significantly reduced Amoxicillin induced bacterial endotoxin than ?-lactam. The other two drugs ciprofloxacin-induced bacterial endotoxin than quinolones increased significantly. When E. coli and Salmonella in different concentrations of antibacterial drugs in the 0.5MIC when ?-lactams and quinolones induce bacteria release toxins more, aminoglycoside-induced release of bacterial endotoxin moderate amount, and 5MIC, 10 MIC are less. With the prolongation of incubation time, antibiotics induced the bacteria ebdotoxin release gradually increased.?-lactams, fluoroquinolones and aminoglycosides antibiotic effect on E. coli and salmonella, two antibacterial agents more than before the latter antibiotic induced release of endotoxin. In addition, the reason why these three antibacterials act on E. coli and salmonella, the endotoxin-induced release of different types of action time of its causes and antimicrobial agents, the concentration of antimicrobial drugs and antimicrobial drugs related. So in addition to considering clinical drug sensitivity of the bacteria and the toxic side effects of drugs, try to choose induce release toxins fewer drugs to achieve better therapeutic purposes.