| Objective:To observe the impact of Fuzilizhong Clyster on the type deficient of spleen and kidney yang of ulcerative colitis （UC） rats. From the pathological morphology and intercellular adhesion molecules （ICAM）-1 point, we discusses its possible mechanism.Methods:After all the 60 SPF rats were adaptively fed for one week, they were randomly divided into the normal group （10 rats） and the model group （50 rats）. Except the normal group were given distilled water, others were to be prepared 15% Senna Decoction （4ml/each rat） and subcutaneous injection of hydrocortisone （10 mg /kg） for 15 days, to preparing the type deficient of spleen and kidney yang. UC modeling:First remove the model rat back hair and receiving protection At the beginning of the sixteenth day, we drops 2%DNCB acetone liquid 0.3ml of each rat for 14d; Thirtieth day, fixed rats and pump 0.1%DNCB ethanol （50%） solution （0.3ml） into the intestine from the anal about 8 cm. Next day the modeling group were given 0.2%glacial acetic acid solution （2 ml） at the same site. After accurate timing 15s,5 ml of physiological saline were pumped. And the normal group were given equal volume of distilled water, modeling is completed. Three model rats and two normal rat were randomly killed and sent to pathological section, which confirmed the model rats were in accordance with the characteristics of UC. Modeling rats were randomly divided into five groups:model group 10 rats, sulfasalazine Group 8 rats; FuziLizhong Decoction high, medium, low dose group have 8 rats respectively;And at the same time the normal group have 8 rats. After the model was built three days, the FuziLizhong clyster and SASP were given for 15 days from the anal about 8cm (high dose:14.18 g·kg-1; middle dose:7.09 g·kg-1 low dose:3.54 g·kg-1; SASP dose: 0.42g·kg-1). But the normal group and model group were given equal volume of distilled water. After the last administration of 24h, the rats were anesthetized.And the colon were clipped at the Position in the anal 4-10cm. The colon （2.5cm* lcm） of rats in each group were placed in 10% neutral Formaldehyde Solution to prepare pathological examination. HE staining was performed under light microscope to observe the pathological morphology; the remaining colon tissue were preserved in the liquid nitrogen. The ICAM-1 expression in rat colonic tissues were detected by immunohistochemical staining （SP method） and Western blot. （WB）.Results:①Pathological morphologic change:compared with the model group rats pathological score （2.30±1.06）, FuziLizhong Decoction high、middle、low dose group colon mucosa pathological scores were significantly decreased. They were （1.00±1.31） （1.63±1.06） （1.13±0.99） （P<0.05）; And the curative effect of the high dose group of the Fuzilizhong clyster is higher than the S ASP group （1.13±1.55）, the difference was statistically significant（P<0.05）.② The ICAM-1 expression in rat colonic tissues were detected by immunohistochemical staining （SP method）:Compared with the control group （0.2593± 0.0126）, the expression of ICAM-1 in colonic tissue of model group（0.3037 ±0.0253） was significantly higher than the model group with significant statistical difference （P<0.01）; Compared with model group, the ICAM-1 protein of all the group were decreased including high dose group （0.2646±0.0160）, middle dose group （0.2856±0.0123） and SASP group （0.2650 ± 0.0081）.And the difference between groups was statistically significant （P<0.05）;③The ICAM-1 expression in rat colonic tissues were detected by Western blot （WB）:Compared with the blank group （0.11 ± 0.013）, the expression of ICAM-1 protein in colonic mucosal cells of the model group was significantly increased （0.44 ± 0.015） （P<0.05）; Compared with the model group, the ICAM-1 protein expression in colon mucosal cells of all the group were decreased, including FuziLizhong high dose Decoction（0.29±0.016）,middle dose group（0.33±0.014）,low dose group（0.31±0.012） and Western medicine group （0.34±0.090）.And the effect of Fuzilizhong Clyster high dose is better than western medicine group, the difference was statistically significant （P<0.05）.Conclusion:1. Fuzilizhong Clyster can improve the overall symptoms and pathological score of the UC rats with the type deficient of spleen and kidney yang. It has protective effects on the intestinal mucosa.2. The Fuzilizhong decoction can reduce the expression of ICAM-1 in colon tissue of the UC rats with the type deficient of spleen and kidney yang and thus play a role in the treatment of UC. |
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