Association Study Between Single Nucleotide Polymorphism Loci And Papillary Thyroid Cancer

Objective: Thyroid cancer is the most common malignant tumor of the endocrine system,the majority of differentiated thyroid carcinoma is thyroid papillary carcinoma.It is reported that SNP rs965513?rs944289?rs10136427?rs7267944?rs966423?rs1801516?rs2439302?rs925255?rs2910164 9 loci are associated with PTC,but there has not reported whether these9 loci have a correlation with PTC in northern China,then the purpose of this study is whether verify the relevance of these sites and PTC in Jining area,we will research the relationship between the four risk factors(tumor size,exposure history,family history,iodized salt consumption)with PTC loci.Methods: We Collected blood samples from July 2015 to July 2016 in Shandong province Jining medical college affiliated hospital a surgical treatment of a total of 103 cases of papillary thyroid carcinoma patients.All patients were from areas in the north of China,mainly the Jining and the surrounding counties and cities area.The standard of the case group is: he had not received the thyroid surgery treatment or drug.The controls come from the examination center of Jining medical college affiliated hospital in Shandong province,100 cases of healthy people,who need registration and match with PTC patients in age?sex or body mass index.The selection criteria of control group include: there is no history of cancer,there is no history of thyroid disease,without history of radiation exposure,there is no history of hypertension,metabolic diseases,etc.The 10 ml venous blood collected on empty stomach at early morning and put on ethylene diamine tetraacetic acid(EDTA)anticoagulant tube,shake up and down then put in the 4? refrigerator experiment,then extracted genomic DNA in small quantities by using blood genome pillar extraction kit.We genotype nine SNP loci(rs965513?rs944289?rs10136427?rs7267944?rs966423?rs1801516?rs2439302?rs925255?rs2910164)for 103 patients and 100 healthy people in the north of China by using the 3730 xl platform of SNP SNapshot technology-multiple single base extension system.Result: 1.we are successful in the genotype of SNP loci.2.There have a significant correlation between the risk of PTC and rs7267944 or rs2910164 by Chi-square test or Fisher test(P<0.05).The genotype or allele frequency distribution of rs965513?rs944289?rs966423?rs2439302?rs10136427?rs1801516 SNP loci have no statistical differences between the casegroup and control group(P<0.05).3.Age composition or gender composition in the case group and the control group has no statistical sense(P>0.05).4.We made a further analysis according to the high risk of thyroid cancer risk factors(radiation exposure history,family history of tumor size,and iodized salt consumption)and the results showed that: the GG genotype or G allele of rs965513 interact with family history(P<0.05).The GG genotype of rs966423 interact with radiation exposure(P<0.05).There are interactions between the TC genotype of rs7267944 loci and family history of TC(P<0.05).There are interactions between rs2910164 loci and TC four risk factors for radiation exposure,tumor size,family history,and high iodine diet(P<0.05).Conclusion: 1.The genotype or allele of SNP rs7267944 and rs2910164 may be associated with PTC in east China.There has no connection between genetic susceptibility of PTC and the allele or genotype of rs965513,rs944289,rs966423,rs2439302,rs10136427,rs1801516.2.The genotype GG or allele G of SNP rs965513 and the genotype TC of rs7267944 may be interaction with family history of PTC.The genotype GG of SNP rs966423 may be interaction with radiation exposure of PTC.Rs2910164 all have interaction with four risk factors(radiation exposure?tumor size?family history and high iodine diet)of PTC.