Treatment Of Pelvic Organ Prolapsed By Tissue Engineered TGF?-1 Incorporated With Adipose Derived Stem Cell Through Reversing The Pathological Environment

Pelvic organ prolapse(POP)is a common disease in the middle and old aged women.There is still a lack of etiological treatment for this disease.Our results showed that there was a disordered arrangement of the elastic fibers and a decreased expression of the elastin gene in the pathological sites of vaginal tissue compared with the normal sites from POP patients,this implied that the destruction of the pelvic floor microstructure may be the pathological factors of POP.Three animal models were built and compared according to the pathological changes found in POP patients and the results showed that the loxll(loxll plays an important role in elastin synthesis and cross linking)knockout mice with a phenotype of rectal prolapse,and their synthesis and arrangement of elastic and collagen fibers were also disordered in their pelvic floor.So the loxll gene knockout mice could be used as an animal model.This study aims to regulate the behaviors of stem cells to increase extracellular matrix synthesis through tissue engineering technology.A silk fibroin microspheres were fabricated by incorporating with ADSCs(adipose derived stem cells)treated with TGF?-1.The results showed that TGFp-1 could increase the expression of LOX family members and elastin from ADSCs cultured in silk fibroin microspheres.At last,the tissue engineered silk fibroin microspheres were injected into the pelvic floor of model mice to increase the synthesis and cross-linking of the elastin,then to reverse the symptom of prolapse.This study would provide new ideas for the clinical treatment of POP.