Effect Of Panax Notoginsenosidumon MiR-130a-3p And Signaling Pathway In Newborn Mice Induced By Oxygen Retinopathy

Objective: To study the effect of Panax Notoginsenosidum vascular lesions in oxygen induced retinal neovascularization,Panax Notoginsenosidum confirmed that promoting blood circulation and removing blood stasis had a good therapeutic effect on oxygen induced retinopathy in rats,to explore the mechanism of inhibitory effect of Panax Notoginsenosidum on oxygen induced retinopathy in neonatal mice for treatment of retinopathy of prematurity(ROP)to provide new drugs.Methods: Using the Smith modeling method of high concentration oxygen to establish pathological changes of retinopathy of prematurity model induced by neonatal mice,were randomly divided into five experimental animal groups: normal control group(A)oxygen induced model group(OIR)(B);intraperitoneal injection of 4 ?L group(C);intraperitoneal injection of 8?L group(D);intravitreal injection of 1 ?L group(E)(n=15)B,C,D,E group using oxygen induced retinopathy reform model,including C?D group were intraperitoneally injected corresponding dose Panax Notoginsenosidumat P7-P12 a day.In group E,Panax Notoginsenosidum was injected into the vitreous cavity at P12(1 ?L).P17,all rats were used for heart perfusion,the fluorescein isothiocyanate(FITC)method for retinal flatmount to observed the retinal vascular morphology,retinal endothelial cell nuclei by HE staining to highlight the inner limiting membrane,the differential expression of Real-time detected by PCR related genes: mi R-130a-3p and its target gene protein kinase D3(Prkd3),VEGFand EGER signaling pathway is involved in the nuclear transcription factor(NF-?B)?Prkd3? VEGFA,epidermal growth factor receptor(EGFR)expression,and Panax Notoginsenosidum of insulin-like growth factor-1 expression(IGF-1).Vascular endothelial growth factor(VEGF)immunohistochemistry to observe the positive expression of VEGF.Results: HE staining showed that C,D,E group than in the OIR group,the number of endothelial cells in the inner limiting membrane was reduced,and the E group was significantly reduced,the difference was significant(P < 0.05).The expression of VEGF in E group was the least in VEGF group.The expression of mi R-130a-3p and IGF-1 were significantly different(P < 0.05).The expression of NF-kappa B,Prkd3,VEGFA and E in the VEGF signaling pathway was significantly different(P < 0.05).There were significant differences in the expression of NF-?B,Prkd3 and EGFR in the EGFR signaling pathway(P < 0.05).Conclusion: the total saponins of Panax Notoginsenosidumcan effectively inhibit OIR mice retinal vascular newborn,improve vascular morphology distortion,hyperplasia,non perfusion phenomenon;and can affect the expression of OIR in gene expression;expression of mi R-130a-3p was down regulated in the after treatment,the expression also raised its target gene Prkd3;expression of VEGF signaling pathway PKC in OIR rats retina VEGFA can downregulate the expression of PKC in EGFR signaling pathway.