| Objective: Gastric cancer is a common malignant tumor with high incidence and mortality rate.Epithelial-mesenchymal transition(EMT)plays an important role in tumor progression by promoting invasion and metastasis.Quercetin,a member of flavonoids family that widely distributes in various fruits and vegetables,is proved to be a potent antioxidant which has exhibited efficient inhibition activity against inflammatory,angiocardiopathy and tumor.Previous studies have showed the reduction effect of quercetin on cancer cell adhesion,angiogenesis,invasiveness and metastasis.This study aimed to explore the effect of quercetin on epithelial-mesenchymal transition in gastric cancer SGC-7901 cells and its possible mechanisms.Methods: Gastric cancer SGC-7901 cells were cultured and divided into three groups,i.e,control group(normal culture medium),LY294002 group(normal culture medium,LY294002),Quercetin group(normal culture medium,Quercetin).The proliferation inhibition of gastric cancer cells by quercetin and LY294002 was examined by MTT assay.Scratch wound assay was used to detect the invasion and migration levels of the cancer cells.The expressions of AKT,p-AKT,Snail,Vimentin and E-cadherin were measured by Western blot and q PCR methods.Results: Quercetin and LY294002 both inhibited the proliferation of gastric cancer cells obviously,and the 50% inhibitory concentration(IC50)of quercetin was(112.9±2.05) ?mol/L,and(46.35±3.13)?mol/L for LY294002.The cell migration distances of the quercetin group((0.15±0.02)mm)and the LY294002 group((0.16±0.03)mm)were significantly higher than that of the control group((0.44±0.04)mm),indicated that quercetin can inhibit the proliferation and migration of gastric cancer cells.The cell migration distances between the quercetin group and the LY294002 group showed no significant difference.Compared with the control group,the expressions of epithelial mesenchymal transition related factors of LY294002 group and Quercetin group were different,the expressions of Snail and Vimentin m RNA and protein in LY294002 group and Quercetin group were down-regulated,while E-cadherin m RNA and protein in LY294002 group and Quercetin group was up-regulated.In the meanwhile,the expressions of p-Akt protein of LY294002 group and Quercetin group were both lower then the control group.The expressions of Snail?Vimentin and E-cadherin m RNA and protein and p-Akt protein between the quercetin group and the LY294002 group both showed no significant difference.Conclusion: Quercetin can inhibits the epithelial mesenchymal transition of human gastric cancer SGC-7901 cells,and its mechanism is probably achieved by inhibiting the AKT signaling pathway. |
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