The Experimental Research On The Influence Of LC-1 Of HSP70 And PRDX1 Expression On Lewis Lung Cancer Mice

Objective: The animal models in this experiment are Lewis lung cancer mice.Immuno-histochemical method is adopted to detect the expression condition of PRDX1 and HSP70 protein in the tumor tissue of Lewis lung cancer mice.Study the anti-cancer principle of LC-1 to provide a reliable scientific basis for the clinical application and promotion of this drug.Methods: Lewis lung cancer model was copied and 48 successfully copied Lewis Lung cancer SPF level C57 mice were randomly divided into 4 groups:tumor-bearing control group,LC-1 group,DDP group and combination treanmnt group.Mice in the tumor-bearing control group were given normal saline lavage once a day,celiac injection of saline solution every three days;mice in the LC-1group were given LC-1 liquid lavage once a day,celiac injection of saline solution every three days;mice in the DDP group were given saline lavage once a day,celiac injection of cis-platinum every three days;mice in the combination treanmnt group were given LC-1 liquid lavage once a day,celiac injection of cis-platinum every three days.Mice of all experimental groups were killed by breaking the neck on the13 th day.Tumor specimens were taken out to weigh the tumor weight and calculate the anti-tumor rate;after the tumor specimens were fixed,section staining was adopted to conduct histopathologic examination and Immunohistochemical Sp method was adopted to test positive expression of HSP70 and PRDX1.SPSS 22 software was adopted to conduct data statistical analysis.Results:1.Tumor weight and anti-tumor rate determination:1.1 Tumor weight: comparing the mean tumor weight(7.28 ± 0.78g)of tumor-bearing control group,the mean tumor weight of LC-1 group(6.21 ± 0.83g)was lower than that of this group and the difference had statistical significance(P<0.05);the mean tumor weight of DDP group(5.34 ± 0.66g)was lower than that of this group and the difference had statistical significance(P<0.01);the mean tumor weight of combination treanmnt group(4.24 ± 0.08g)was also lower than that of this group and the difference had statistical significance(P<0.01).Taking LC-1group as the comparison object,the mean tumor weight of DDP group was lower than that of this group and the difference had statistical significance(P<0.05);the mean tumor weight of combination treanmnt group was lower than that of this group and the difference had statistical significance(P<0.01).Taking DDP group as the comparison object,the mean tumor weight of combination treanmnt group was lower than that of this group and the difference had statistical significance(P <0.01).1.2 Anti-tumor rate determination: the anti-tumor rate of combination treanmnt group was 41.8%,which was the best;the anti-tumor rate of DDP group was second,26.6%;the anti-tumor rate of LC-1 group was the lowest,14.7%.2.HSP70 expression examination: the mean of aptical density of HSP70:tumor-bearing control group(0.0141 ± 0.0018),LC-1 group(0.0108 ±0.0011),DDP group(0.0101 ± 0.0007),and combination treanmnt group(0.0071 ± 0.0013).The HSP70 expression the mean of aptical density of LC-1group was lower than that of tumor-bearing group and the difference had statistical significance(P < 0.01);HSP70 expression the mean of aptical density of DDP group was lower than that of tumor-bearing group and the difference had statistical significance(P < 0.01);HSP70 expression the mean of aptical density ofcombination treanmnt group was lower than that of tumor-bearing group and the difference had statistical significance(P < 0.01);HSP70 positive expression the mean of aptical density of LC-1 group was higher than that of DDP group but the difference had no statistical significance(P>0.05);HSP70 positive expression the mean of aptical density of LC-1 group was higher than that of combined drugs group and the difference had statistical significance(P<0.01);HSP70 positive expression the mean of aptical density of combination treanmnt group was lower than that of DDP group and the difference had statistical significance(P<0.01).3.PRDX1 expression examination: the mean of aptical density of PRDX1 protein positive expression: tumor-bearing control group(0.0128 ± 0.0017),LC-1 group(0.0100 ± 0.0015),DDP group(0.0090 ± 0.0007),and combination treanmnt group(0.0060 ± 0.0013).PRDX1 expression,the mean of aptical density of LC-1 group was lower than that of tumor-bearing group and the difference had statistical significance(P<0.01);PRDX1 expression the mean of aptical density of DDP group was lower than that of tumor-bearing group and the difference had statistical significance(P<0.01);PRDX1 expression the mean of aptical density of combination treanmnt group was lower than that of tumor-bearing group and the difference had statistical significance(P<0.01);PRDX1 positive expression the mean of aptical density of LC-1 group was higher than that of DDP group but the difference had no statistical significance(P>0.05);PRDX1 positive expression the mean of aptical density of LC-1 group was higher than that of combination treanmnt group and the difference had statistical significance(P <0.01);PRDX1 positive expression mean value of combination treanmnt group was lower than that of DDP group and the difference had statistical significance(P<0.01).4.Microscopic observation of tumor pathology: comparing the three treatment groups and the tumor-bearing control group,the necrosis area of tumor cells increased and enlarged,presenting spot or flake type,the cell edema increased,the cell density decreased relatively,the atypia of cells decreased and the pathologicmitosis decreased;comparing treatment groups,combination treanmnt group had the most evident effect while DDP group had more evident effect than LC-1 group.Conclusions:1.LC-1 can reduce the expression of Lewis lung cancer PRDX1 and HSP70,which is related to the tumor cell's apoptosis,having the effect of anti-cancer.2.DDP can reduce the expression of Lewis lung cancer PRDX1 and HSP70,which is related to the tumor cell's apoptosis,having the effect of anti-cancer.3.Comparing single use of LC-1 or cis-platinum and the combination of LC-1and cis-platinum,the combination of LC-1 and cis-platinum is superior to the single use of LC-1 or cis-platinum in reducing Lewis lung cancer PRDX1 and HSP70 expression of mice,indicating that combination of LC-1 and cis-platinum has a higher anti-cancer effect.