The Expression Of TCF7L2 In Gastric Carcinoma And Its Relationship With The Occurrence And Development Of Gastric Carcinoma

Background and Objectives: Gastric cancer is one of the most common malignancies in the world.The incidence and mortality of gastric cancer in China are among the highest in malignant tumors,which seriously endangers human health.Wnt signaling pathway is one of the key pathways in the development of vertebrate embryos.The abnormal activation of Wnt signaling pathway is closely related to the occurrence and development of various tumors.The study found that in the development of gastric cancer also appeared the Wnt signal pathway abnormalities.Transcription Factor 7-Like2(TCF7L2)is an important regulatory factor in the Wnt signaling pathway,and its overexpression can cause abnormal regulation of the Wnt signaling pathway.In this study,TCF7L2 as the research object,to explore its expression in gastric cancer and the occurrence and development of gastric cancer.Materials and Methods: The amount of TCF7L2 m RNA was detected by RT-qPCR in15 cases of gastric cancer,adjacent tissues and gastric cancer cell lines.Western blot was used to detect the expression of TCF7L2 protein in gastric cancer tissues,gastric cancer tissues and gastric cancer cell lines.The SGC-7901 / TCF7L2 cell lines with high expression of TCF7L2 were constructed by stable plasmid transfection technique.The cell morphology of SGC-7901/TCF7L2 and SGC-7901 cell lines was observed and compared under light microscopy.Wound Healing to compare the ability of migration between the two cell lines,;In vitro,tumorsphere assay to compare the ability of into a ball between the two cell lines;The human gastric cancer cell lines SGC-7901/ TCF7L2 and SGC-7901 were injected into the nude mice which are 4-5 weeks old,to establishthe models of nude-mice bearing tumor,the tumor volume was measured twice a week,and the effect of TCF7L2 on the growth of gastric cancer bearing tumor was observed.Results: The expression level of TCF7L2 in gastric carcinoma was significantly higher than that in surrounding tissues,and the expression level of TCF7L2 in gastric cancer cell lines was significantly higher than that in normal gastric epithelial cells(GES-1);The stable transfection technique successfully established stable SGC-7901/TCF7L2 cell lines,which the expression of TCF7L2 mRNA and protein were significantly higher than that of SGC-7901 cell line.The cell morphology of SGC-7901/TCF7L2 was diversity than that of control cell,and the number of pseudopodia increased,the volume of granules increased,the ability of migration,the ability of into a ball and the resistance of platinum drugs than in control cells significantly enhanced than that of control cells;The tumorigenic ability of SGC-7901/TCF7L2 cells in mice was also significantly enhanced.Conclusion: The expression level of TCF7L2 in gastric carcinoma and in gastric cancer cell lines were both significantly higher than the control groups,which indicated that TCF7L2 might be associated with the development of gastric cancer.The cell morphology of SGC-7901/TCF7L2 was diversity than that of control cell,and the number of pseudopodia increased,the volume of granules increased,the ability of migration,the ability of into a ball and the resistance of platinum drugs than in control cells significantly enhanced than that of control cells,which indicated that in development of gastric cancer,TCF7L2 changes the shape of gastric cancer cells,promotes its invasion and proliferation,showing the malignant transformation of the tumor;the two models of nude-mice bearing tumor,and the results showed that TCF7L2 could promote the growth and proliferation of gastric cancer cells in vitro and further verify the results of in vitro experiments.