Preliminary Study On The Reaction Between Carotid Atherosclerotic Unstable Plaque And Bile

Objectives:To study the dissolution of carotid atherosclerotic plaque in acidic condition and the occurrence of bile, analyze the changes of plaque composition before and after the reaction.Methods:The first part, Carotid atherosclerotic plaques were collected by carotid endarterectomy,and were classified into unstable plaques and stable plaques according to the composition of the plaque, each type of plaque was divided into three groups. Bile (PH≈7),dilute hydrochloric acid (PH=2), bile after acidification (PH=2) were added under the experimental conditions at 25 ℃, the dissolution of each group was observed and recorded.The second part, the change of plaque composition before and after the reaction was studied by gas chromatography combined with mass spectrometry. The lipid necrosis core of the pre-reaction precursors and the dark green flocculent substances formed after the reaction were measured respectively.The third part, the changes in protein composition before and after the reaction were measured using Coomassie brilliant blue method combined with mass spectrometry. The protein samples were extracted before and after the reaction, and the contents of the proteins in the samples were compared before and after the reaction.Results:1. Only acidified bile (PH = 2) can undergo a significant dissolution reaction with carotid atherosclerotic instability plaques, dilute hydrochloric acid (PH = 2) and bile (PH = 7)had no significant response to carotid atherosclerotic plaques.2. Only the unstable plaque can undergo a significant dissolution reaction with the bile.3. Unstable plaque and acidification of the bile after the dissolution reaction, we can see the unstable plaque lipid necrosis core in the acidification of the bile after the rapid softening and dissolving, and ultimately into a dark green floc.4. Gas chromatography combined with mass spectrometry can be used to determine the changes of sugar, acid, alcohol, ester and other smaller molecular weight components before and after the reaction. There were 11 kinds of small molecular substances in the core of the lipid necrosis before the reaction, and 50 species were found in the flocculent matter after the reaction.5. Coomassie brilliant blue method combined with mass spectrometry can be measured before and after the reaction of macromolecular protein composition changes. Finally,there were 453 proteins in the nucleus of the lipid necrosis before the reaction, and there were 191 species in the reaction, of which 285 were eliminated and 60 new.Conclusions:1. Carotid atherosclerotic instability plaque and acidic conditions of bile (PH = 2) occurred is the plaque in the lipid necrosis of the core and acidification of the bile reaction, the final softening dissolved into an ink Green flocculent material.2. Lipid necrosis Core volume of the larger unstable plaque in the acidification of the bile after the dissolution of significant effect, this phenomenon is expected to unstable plaque vascular intervention therapy to provide a new method.3. Gas chromatography combined with mass spectrometry can be used for lipid necrosis of the core and the reaction after the formation of dark green flocculent sugar, acid, alcohol,ester and other small molecular substances in the comparative determination.4. The technique of Coomassie brilliant blue method combined with mass spectrometry can be applied to the determination of macromolecule protein components.5. The lipid-necrotic core of the unstable plaque and the composition of the flocculent material formed after the reaction are extremely complex. The key mechanism of studying the response of the carotid atherosclerotic plaque to the bile under acidic conditions is a complex Work, need to go through a lot of experiments, modify, adjust,and the need for neurosurgery, hepatobiliary surgery, biochemistry experts and other disciplines of researchers in close cooperation.