The Correlation Study Of Syndrome Differentiation Of Traditional Chinese Medicine, Clinicopathological Features, And The Expression Of MiR-21 And MiR-197-3P Related To Non-small Cell Lung Cancer

Objective:To investigate the correlation of syndrome differentiation of traditional Chinese medicine(TCM),clinicopathological features,and the expression of miR-21 and miR-197-3p related to non-small cell lung cancer(NSCLC).Methods Screening 60 cases of NSCLC patients according to the selection criteria,and making clear pathological type,clinical stage,and syndrome type of TCM according to the clinical observation Tab of lung cancer.The expression levels of miR-21 and miR-197-3p in 60 cases of primary NSCLC patients and 20 healthy population were detected by fluorescence quantitative PCR method,and the diagnostic value of miR-21 and miR-197-3p in NSCLC patients was analyzed.The data of clinical observation Tab and experimental data were collected and analyzed statistically.Results1 A total of 60 cases of NSCLC patients were enrolled in this study.In the distribution of gender,37 cases of male(61.67%),23 cases of female(38.33%),the ratio of male to female was 1.61:1;in the distribution of pathological type,26 cases of squamous cell carcinoma(43.33%),34 cases of adenocarcinoma(56.67%);in the distribution of clinical stage,1 cases of stage I(1.67%),3 cases of stage II(5%),20 cases of stage III(33.33%),36 cases of stage IV(60%).Qi deficiency and phlegm dampness type is the most common type of TCM syndrome in lung cancer,accounting for 43.33% of the total number of cases,followed by qi and yin deficiency type,accounting for 21.67%,and qi stagnation and blood stasis type,yin deficiency and toxic heat type,and phlegm heat in the lung type,accounting for15%,11.67%,8.33%,respectively.2 The analysis of the distribution of TCM syndromes in different gender showed that men were slightly more than women in all TCM syndromes,and difference was not statistically significant,indicating that there was no difference in the distribution of TCM syndromes in patients with NSCLC.There was no significant difference in the distribution of TCM syndromes in different pathological types,indicating that there were no differences between adenocarcinoma and squamous cell carcinoma in NSCLC patients.There was no significant difference in the distribution of TCM syndromes in different clinical stages,and the TCM Syndromes of patients with stage III were similar to stage IV in NSCLC patients,and Qi deficiency and phlegm dampness type is the common syndrome of TCM.3 The statistical analysis of the relative expression of two miRNA in NSCLC group and normal control group,showed that relative expression of miR-21 and miR-197-3P in whole blood of NSCLC group were higher than the normal control group(P < 0.01),and the difference was statistically significant.The study compared the diagnostic value of miR-21 and miR-197-3p in patients with NSCLC,displayed that the combination of the two indicators can distinguish NSCLC patients from the normal control group with high diagnostic value,and the sensitivity,specificity,positive predictive value,the negative predictive value was 92%,80%,93.5%,88.9%,respectively.Correlation analysis between the expression level of miR-21 of 60 cases with NSCLC and clinicopathological features,showed that the expression level of miR-21 has no significant correlation with gender(P=0.55),smoking history(P=0.748),pathological type(P=0.606)and TCM syndrome(P=0.638).The expression level of miR-21 was significantly correlated with clinical stage(P=0.000).Correlation analysis between the expression level of miR-197-3p of 60 cases with NSCLC and clinicopathological features,showed that the expression level of miR-197-3p has no significant correlation with gender(P=0.482),smoking history(P=0.587),clinical stage(P=0.311)and TCM syndrome(P=0.917).The expression level of miR-197-3p was significantly correlated with pathological type(P=0.01).Conclusion1 The pathological types and clinical stages of NSCLC patients has no significant correlation with TCM syndrome.2 There was no correlation between gender and TCM Syndromes in NSCLC patients;There was no correlation between adenocarcinoma,squamous cell carcinoma and TCM Syndromes;the TCM Syndromes of patients with stage III were similar to stage IV in NSCLC patients,and Qi deficiency and phlegm dampness type is the common syndrome of TCM.3 Results showed that both miR-21 and miR-197-3p were up-regulated in patients with NSCLC,and can be used as biomarkers for the diagnosis of NSCLC patients with new material,and the combined diagnosis has high diagnostic value.The expression level of miR-21 was significantly correlated with clinical stages,while no significant correlation with gender,smoking history,pathological type,and TCM syndrome.The expression level of miR-197-3p was significantly correlated with pathological type,while no significant correlation with gender,smoking history,clinical stages,and TCM syndrome.