TIP30 And OPN Expression In Oral Squamous Cell Carcinoma And The Influence Of Up-regulated Their Expression In Cal-27 Cells

TIP30 and OPN expression in oral squamous cell carcinoma and the influence ofup-regulated their expression in cal-27 cells Objective:To observe the TIP30 and OPN expression in oral squamous cell carcinoma,to study the influence of the biological behavior of raised OPN or TIP30 in cal-27 cell lines.Methods:The expression of OPN and TIP30 protein were examined in OSCC(n=14),in adjacent tissues(n=14)and in normal oral mucosa(n=8)by the method of western blot.The TIP30 and OPN were raised to cal-27 cell lines respectively by infection or transfection technology,respecetively.Respectively,real-time fluorescent quantitative PCR(RT-PCR)and western blot methods were used to detect TIP30 and OPN expression in cell lines.The proliferation ability of cal-27 cell lines was tested by CCK-8 tests,while the migration ability of them was tested by cell scratch experiment tests.The results were analysised by SPSS 20.0 software.Results: 1.Comparison of expression of OPN protein in OSCC,adjacent tissues and normal oralmucosa.Using the method of one-way analysis of variance(ANOVA)and LSD-t to analyze the results.The expression of OPN protein in OSCC is higher than adjacent tissues and normal oral mucosa(P<0.001).It is still cannot considered that there is a difference between the expression of OPN proteins in adjacent tissues and normal oral mucosa(P=0.592).2.Comparison of expression of TIP30 protein in OSCC,adjacent tissues and normal oralmucosa.Using the method of one-way analysis of variance(ANOVA)and LSD-t to analyze the results.The expression of TIP30 protein in OSCC is lower than adjacent tissues and normal oral mucosa(P<0.001).It is still cannot considered that there is a difference between the expression of TIP30 proteins in adjacent tissues and normal oral mucosa(P=0.540).3.Detection of the expression of OPN mRNA and protein in up-regulated cal-27 celllines.Using the method of paired t-test to analyze the results.The expression of OPN mRNA and protein in cal-27-shOPN cell line is higher than in cal-27-shN cell line(P<0.001,P=0.002).4.Detection of the expression of TIP30 mRNA and protein in up-regulated cal-27 celllines.Using the method of paired t-test to analyze the results.The expression of TIP30 mRNA and protein in cal-27-pTIP30 cell line is higher than in cal-27-pc3.0 cell line(P=0.001,P=0.003).5.Detection of the proliferation ability of up-regulated OPN in cal-27 cell lines.Using the method of multiple variance analysis to analyze the results.The proliferation ability of cal-27-shOPN cell line is better than cal-27-shN cell line(P<0.001).6.Detection of the proliferation ability of up-regulated TIP30 in cal-27 cell lines.Using the method of multiple variance analysis to analyze the results.The proliferation ability of cal-27-pTIP30 cell line is worse than cal-27-pc3.0 cell line(P<0.001).7.Detection of the migration ability of up-regulated OPN expression in cal-27 cell lines.Using the method of multiple variance analysis to analyze the results.The migration ability of cal-27-shOPN cell line is better than cal-27-shN cell line during 24h(P=0.002).8.Detection of the migration ability of up-regulated TIP30 expression in cal-27 cell lines.Using the method of multiple variance analysis to analyze the results.The migration ability of cal-27-pTIP30 cell line is worse than cal-27-pc3.0 cell line during 24h(P=0.005).Conclusions:In OSCC,the expression of OPN is higher while the expression of TIP30 is lower.Up-regulated OPN expression of cal-27 cell line can improve the ability of proliferation and migration,while up-regulated TIP30 expression of cal-27 cell line can reduce the ability of proliferation and migration.OPN expression in OSCC may participate in the occurrence and development of tumor,while TIP30 may have the opposite effect in OSCC.