| This thesis includes two parts,the study on three effective components assay and HPLC fingerprint of Laggerae herba the content determination of saponins in Panax notoginseng.The L9(34)orthogonal experiment design was used for optimization of extraction method for the three active components,Ilicic acid,Artemitin,Pterodontic acid in laggerae herba.On the basis of single factor experiments,extraction time,ratio of solid to liquid,fraction of solution volume were chosen to study the effects of interaction of various factors on the extraction rate of the three active components.According to the results,the optimum extraction method for the three active components was as follows:the extraction time was 40mins,the ratio of raw material to solvent was 1:4(g:mL),the fraction of solution volume was 90%.29 Batches of laggerae herba samples collected in September from different areas in Yunnan Dehong,and 9 batches of laggerae herba samples collected at different times from the same place in Yunnan Mang City were determined,using standard curve.The results showed that the herbs of white stems was relatively higher in content comparing with the purple stems,and the content of the three active components varied widely as a result of different growth environment and different picking time.The sample picked in November had the highest content in all the three active components.Four batches of laggerae herba samples were analyzed by "quantitative analysis of multi-components by single-marker".Compared the results came from the standard curve to that of "quantitative analysis of multi-components by single-marker" method,there was only substances with the similar structure can adopt the "multi-evaluation" method.According to the experience of private medication and reports in the literature,10 batches of laggerae herba were selected to establish a HPLC fingerprint.The established HPLC fingerprint could be used as a standard to evaluate the quality of laggerae herba,the method was reliable,accurate,stable and reproducible.Chosen by HPLC fingerprint of laggerae herba,11 batches of samples were tested in antiviral activity,the results were consistent with each other.While compared with the Chinese Pharmacopoeia's just relying on the content of Artemitin as the basis for judging,the fingerprint had its advantages of integrity and ambiguity.Combined with the determination of the content of the three active components,it provided a certain scientific basis for the quality control standards of laggerae herba.According to the results,the draft of the standard of laggerae herba was made.The L9(34)orthogonal experiment design was also used to optimize the extraction method of saponins in Panax notoginseng.According to the result,the optimum extraction condition was as follows:the ultrasonic extraction time was 30min,the ratio of material to liquid was 1.0g/50ml,and the volume fraction of methanol solution was 60%.The content of saponins in 21 batches of sample from different areas of Yunnan Province was determined by high performance liquid chromatography(HPLC),and the quality of Panax notoginseng was evaluated.The results showed that the content of the three main saponins from 21 batches of Panax notoginseng in Yunnan Province all reached the pharmacopoeia requirements and had good clinical application value.Among them,the content of saponins in the sample from Yunlong Dali was relatively higher and the total saponin content was also the highest,the sample had a high application value.The resolution of Rgi and Re got baseline separation,using self-made HPLC column.Six saponins were assayed from 21 batches of Panax notoginseng in Yunnan Province. |
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