Electrodeposition Of Drug Loaded Ethosomes/Silk Fibroin Nanofibrous Composite For Transdermal Drug Delivery And Characterization

Electrospun silk fibroin(SF)nanofibers not only own excellent biocompatibility and skin affinity,but also have attracted much attention in the field of transdermal drug delivery due to their high specific surface area and high porosity.Ethosomes(Eth)were a good drug carrier with good biocompatibility and strong transdermal property.Electrochemical deposition technology was easy to control,and the deposition layer was uniform and smooth.In this work,SF nanofibrous matrices served as template,onto which the Dox-loaded ethosomes(Dox@Eth)were deposited by electrodeposition for transdermal drug delivery system with a series of characterization and evaluation.The main research contents and results are as follows:(1)Preparation and characterization of cationic Eth.The vesicles with uniform size(114.9 ± 6.6 nm)and single layer were prepared by thin film dispersion method,positively charged +39.0 V,and the encapsulation efficiency was up to 82.85%.The Eth was confirmed good cell compatibility.(2)Preparation and characterization of drug loaded Eth/SF composite nanofiber membrane.The SF nanofiber membrane was prepared by electrospinning,and the tensile strength of the fibers increased significantly after cross-linking by EDC/NHS/HA.The SF nanofibrous membrane served as template,onto which the Dox-loaded ethosomes(Dox@Eth)were deposited by electrodeposition.The experimental results shows that the nanocomposite film with good cell compatibility.(3)Study on transdermal drug delivery.The Dox@Eth/SF composite nanofiber membranes carried out by transdermal drug delivery experiment.The results showed that the Dox@Eth could penetrate into the dermis layer across through the stratum corneum.(4)Study on the antitumor properties of Electrodeposited Dox@Eth/SF composite nanofiber membrane.In vitro anti-tumor experiment,the Dox@Eth/SF composite nanofiber membrane prepared by electrodeposition could inhibit the growth of Hela cells effectively,and which was expected to obtain good results in the prevention of postoperative recurrence of superficial tumors.In this study,Dox@Eth/SF composite nanofiber membranes were prepared by electrochemical deposition technique,which not only had the physical and mechanical properties required for transdermal drug delivery patches,but also had a smooth and uniform drug coating.It could be used in the transdermal drug delivery system to allow drugs to enter the deeper layer through the epidermis and effectively distributed in the skin.