The Dynamic Monitoring Of The Chemical Constituents Of Wen Dang Collected In The Different Harvesting Stages And Its Distinguishment From Baitiaodang

Codonopsis Radix,often used in large quantities,is mainly produced in Shanxi,Shaanxi,Gansu,Sichuan provinces and other regions.Wen County in Gansu Province is the genuine producing area of the Wendang.Taking Wendang as the research object,on the basis of the quality formation,medicinal materials origin and characterization of Wendang,this paper mainly focused on the pharmaceutical effective constituents,Wendang contains as the main line,and determined the total saponins and the total flavonoids of Wendang collected from different harvesting stages by the ultraviolet-visible spectrophotometry,and measured the lobetyolin and the syringin by the high performance liquid chromatography.Through the dynamic monitoring of the main chemical composition of Wendang,this article explores the regular pattern of the content of each chemical component of Wendang changing with the season change,so as to provide a more effective data base for the quality evaluation of Wendang,and to provide a scientific basis for further explaining the quality formation.The main research contents and results are as follows:1.Through the determination of different month total saponin content to study the quality of wendang formation.According with 2015 Chinese Pharmacopoeia,ginsenoside content determination,the root of wendang extraction with methanol ultrasonic extraction of methanol,The residue was dissolved in distilled water and extracted with n-butanol.After extraction of the n-butanol,the residue was equilibrat-ed with methanol.The solution was evaporated at low temperature,and 1% ovanilla Aldehyde-glacial acetic acid 0.5m was added,adding perchloric acid 0.5mL,standing at 60? for 30 min,cold bath for 2min,add 5mLof glacial acetic acid,the absorbance was measured at 540 nm and the total saponin content was calculated.The content of total saponins in wendang was fluctuated between 16.4 and 38.56mg/g,and the content of total saponins was the highest between August and September.The content of total saponin in wendang was affected by temperature and season.The most vigorous growth season was the highest period in August and September.2.The quatite of flavonoids in different months was studied to determinewendang's quality.Ultrasonic extraction with 75% ethanol,extract the capacity after,take 2 mL in the 25 mL volumetric flask,After adding 5% Sodium hydroxide,Add10% aluminum nitrate 1 mL,rest for 6 minutes,Adding 5% sodium hydroxide 10 mL,let stand for 15 min color,the measured absorbance at 510 nm,the total flavonoid value in the 15.75~29.37mg/g fluctuations between.The results showed that the content of total flavonoids in wendang showed a trend of decreasing first and then increasing,with the highest content being 7 and 8 months.The content of total flavonoids in wendang was affected by temperature and season,and the accumulation of total flavonoids in wendang accords with the change rule of the flowering period and the growth period.3.Determination of the content of codonopsis pilosula lobetyolin and Syringin with HPLC.Agilent1200 high performance liquid chromatography with a diode array detector(DAD),AT.Lichom C18 liquid chromatography column(250mm×4.6mmid.5um,Lanzhou Zhongkeket);column temperature of 30?.Mobile phase: acetonitrile-0.3% phosphoric acid gradient elution.1~10min acetonitrile 6%,10~20min acetonitrile 16%,20~30min(250mm×4.6mmid.5um,Lanzhou Zhongkekate);column temperature oven 30?,Acetonitrile 26%,30 ~ 40 min acetonitrile 45%,40~45min acetonitrile 45%,45~50min acetonitrile 6%;detection wavelength 267 nm,injection volume 10?L.It was found that codonopsis pilosula lobetyolin and Syringin decreased first and then increased with the seasons,and the content of codonopsis pilosula lobetyolin was the lowest in the most vigorous growing season from July to August.4.Use codonopsis pilosula lobetyolin and Syringin to Identification of baitiaodang and wendang by HPLC.Agilent1200 high performance liquid chromato-graphy with a diode array detector(DAD),AT.Lichom C18 liquid chromatography column(250mm×4.6mmid.5um,Lanzhou Zhongkeket);column temperature of 30 ?.Mobile phase: acetonitrile-0.3% phosphoric acid gradient elution.1~10min acetonitrile 6%,10~20min acetonitrile 16%,20~30min(250mm× 4.6mmid.5um,Lanzhou Zhongkekate);column temperature oven 30?,Acetonitrile 26%,30~40min acetonitrile 45%,40~45min acetonitrile 45%,45~50min acetonitrile 6%;detectionwavelength 267 nm,injection volume 10?L.The results showed that the Syringin were not included in baitiaodang.And the syringin was used as the index to distinguish the baitiaodang and wendang.