| Objective : ulcerative colitis(ulcerative colitis,UC)is a chronic nonspecific inflammatory bowel disease,involving rectum and colonic mucosa and submucosa,having the characteristics of high incidence,recurring easily and long course.We can observe the effects of Weng LianJieDu decoction on the general state of rats,disease activity index(DAI),the content of interleukin-17(IL-17)and the expression of toll-like receptor-2,in order to explore the mechanism of WengLianJieDu decoction to UC rats and try to provide the experimental experience for clinical treatment.Methods : 60 clean and healthy male Wistar rats were divided into 6groups by random number able,10 rats of each group: the blank control group(NG),model group(MG),taking western medicine group(XG),taking ShuangHongQinYuTang decoction groups which includes high concentration(HG)? middle concentration(ZG)and low concentration(LG).The blank control group were fed normally,others were manufactured into ulcerative colitis models by TNBS/ethanol.4 days after modeling,we selected a rat randomly from the group(except NG group),which was beheaded anatomy and we took the heavy colon tissue with naked eye in which the mucosa appeared erosion and ulcers,hyperemia and edema,thus the rats were modeled successfully.In addition to the blank control group and the model group of normal feeding,the rest were treated with corresponding Chinese medicine respectively.XG were gavaged with mesalazine suspension and HG,ZG and LG were gavaged with different concentrations of mixed suspension of ShuangHong Qin YuTang decoction.After continual treatment for 2 weeks,we need observe and note the rats mental state,food consumption,coat color,faeces character and weight of rats in different periods and relying on these to carry on DAI score.At the end of the treatment,each rat of all the groups can only drank water for 24 hours.After abrosia 24 hours,we anesthetized rats with lidocaine,took the femoral arterial blood and detected the content of IL-17 by double antibody sandwich method,at the same time we dissected rats and took colon tissue of the heavier lesion site,which need paraffin embedding,section cutting and HE dyeing to help us test the expression of TLR2.Results: 1 general state: NG group of rats were in good conditions but others' spirits were dropping and reacted slowly,loss of weight and mucous and blood were found in their thin stool.After gavage treatment,the therapy group had good changes which include energetic spirit,good appetite,weight gain and the normal feces in which mucous and purulent disappeared.Among all therapy groups,HG group changed most obviously and restored fastest,XG and ZG had no obvious difference,comparing to HG less poored but better than LG group.DAI score results shows: XG,HG,ZG,LG groups DAI scores were all lower than MG and the differences were statistically significant(P<0.05);HG was lower than XG and ZG,and the difference was statistically significant(P<0.05);LG group compared with XG and ZG,the DAI score was higher,but between XG to ZG there was no obvious statistically significance.(P<0.05);2 The colonic mucosa observation with naked eye and Pathology.The naked eye: the colonic epithelium mucosa of NG group rats was normal.MG rats colonic mucosa was the heaviest in hyperemia and edema and in glands' damages and the most ulcers.HG rats colonic mucosa was smoother and had more new glands and the fewest ulcers than others' groups.XG and ZG rats colonic mucosa were not smooth and had fewer ulcers than LG.LG group rats colonic mucosa were slight hyperemia and edema and had many ulcers.The observation of Pathology: NG group rats mucosa was smooth and the glands were normal.There are many ulcers,broken glands and inflammatory cells in MG group rats rough mucosa.HG group rats mucosa was more smoother and new glands.There were less inflammatory cells and ulcers in XG and ZG groups mucosa.LG group mucosa left more lesions and mild hyperemia and edema and there were some new glands around the ulcers;3 Index detection The comparison of content of IL-17: MG,XG,HG,ZG and LG the content of IL-17 all were higher than NG and the differences were statistically significant(P<0.05);XG,HG,ZG and LG the content of IL-17 all were lower than MG and the differences were statistically significant(P<0.05);XG,ZG and LG the content of IL-17 all were higher than HG and the differences were statistically significant(P<0.05);XG and ZG groups were lower than LG,the differences were statistically significant(P<0.05);XG group and ZG had no statistically significance(P>0.05).The content of TLR-2: the expression of TLR-2 in MG,XG,HG,ZG and LG all were higher than NG and the differences were statistically significant(P<0.05);the expression of TLR-2 in all were lower than MG and the differences were statistically significant(P<0.05);XG,ZG and LG the expression of TLR-2 all were higher than HG and the differences were statistically significant(P<0.05);XG and ZG group were lower than LG,the differences were statistically significant(P<0.05);XG group and ZG had no statistically significance(P>0.05).Conclusions:WengLianJieDu decoction can improve the ulcerative colitis rats general state,restore the damaged colonic mucous and reduce the score of DAI,and the efficacy changes with the different concentration of decoction.The mechanism of the Weng LianJieDu is that the decoction may reduce the expression of TLR-2 and the content of IL-17 in order to block inflammation,reduce the inflammatory cells infiltration,stimulate local tissue regeneration and heal the ulcers. |
PDF Full Text Request