Study On The Resource Chemistry Of Sophora Alopecuroides L.

Objective:This study was designed to evaluate the resource chemical compositions in different officinal portion,different growing time and different areas on the basis of study of chemical compositons in the root and rhizome of Sophora alopecuroides L.,analyze targets and related signal pathways about diabetes and depression based on the ideas of the network pharmacology model,illuminate preliminarily substance basis and molecular mechanism about antidepressant effect and hypoglycemic effects of Sophora alopecuroides L.using pharmacological experiments.Methods:1.Modern chromatographic techniques were adopted to separate and enrich alkaloids and flavonoids in the root and rhizome of Sophora alopecuroides L..2.HPLC-DAD and standard curve method were applied to analyze resource evaluation of chemical compositions,which is separated from Sophora alopecuroides L.,during different officinal portion,phonological perionds and different areas.3.Based on the network pharmacology,we put forward the possible pharmacological effects and mechanisms monomers,which were isolated from Sophora alopecuroides L..4.Using diabetic rat model to determine the hypoglycemic effects of Sophoraflavanone G and total flavonoids of Sophora alopecuroides L..Antidepressant effect of Sophoraflavanone G was observed by measuring changes of behaviors using CUMS model.5.Based on molecular biology techniques,activities study on Sophoraflavanone G and total flavonoids to diabetes and depression related targets and signal pathways will be done.Result:1.Monomer compositions isolated from Sophora alopecuroides L.were(-)-Kurarinone,Exiguachromone A,Trifolirhizin,Sophoraflavanone G,Griffithine.2.5 monomer compositions present certain regularity in different officinal portion,phonological periods and different areas.3.Gene-Drug network graphs of 5 monomer compositions were showed in the paper,and based on the construction of network pharmacology,flavonoid monomer and total flavonoids in Sophora alopecuroides L.had potential to affect insulin signaling pathway and depression signaling pathway.4.Sophoraflavanone G could significantly reduce the levels of blood glucose,insulin and aminotransferase,promote SOD activity in serum,decrease content of MDA,improve pathological condition,reduce the expression of TRX,Txnip and phosphorylation of IKK??IKK??I?B??NF-?B.5.Total flavonoids in the the root and rhizome of Sophora alopecuroides L.could reduce blood glucose and insulin content,obviously relieve pathological condition of liver tissue in rat induced by STZ,lower the expression of aminotransferase in serum and IL-6?IL-1?,TNF-? in liver tissue,increase SOD activity,reduce MDA content,and down-regulate protein expression of P-Akt?P-I?B??P-NF-?B ? P-PI3 K.6.Sophoraflavanone G could significant activity of antidepression,remarkably reduced IL-6,IL-1? and TNF-? level,increased NE and 5-HT content and expression of P-PI3 K,P-Akt,P-mTOR,P-70S6 K,PSD95,GluR1,BDNF and Trkb.Well,blockade of mammaliantarget of rapamycin signaling blunted the antidepressant effects and reversed the up-regulation of BDNF expression caused by SG.Conclusion:1.5 monomer compositions present certain regularity in different officinal portion,phonological periods and different areas.2.It was preliminarily illuminated that total flavonoids in the root and rhizome of Sophora alopecuroides L.exerted antidiabetic effect by PI3K/Akt/NF-?B signaling pathway.3.It was showed preliminarily that Sophoraflavanone G played a role in treating diabetes and interventing complications of diabetes mellitus through Txnip/Trx/NF-?B signaling pathway.4.It was preliminarily revealed that SG alleviated depression-like symptoms via mTOR signal pathway mediated BDNF/Trkb signaling.