Application Of Skin Nerve Biopsy In Peripheral Nerve Injury

Objective:The neuropathic changes of the skin were investigated by skin biopsy technique.The correlation between epidermal nerve fiber density and sciatic nerve function index and nerve conduction velocity was compared.For the assessment of peripheral nerve injury to provide a reference.Methods:A total of 50 rats were randomly divided into two groups.The rats were randomly divided into two groups: control group(n = 10),control group(n = 6)Technique was used to quantify the damaged skin nerve fibers by C5 eyepiece micrometer and Image-pro plus6.0 image analysis software.At the same time,the sciatic nerve function index and nerve conduction velocity were measured at different time points.After the different stages of nerve injury And the degree of recovery of the indicators of the correlation analysis.Results: 1,with PGP9.5 marked nerve fibers,showing that the epidermis is rich in nerve fibers dominated.The normal reference value of IENFD in the dorsal foot of the rat was 20.94 ± 0.34 fibers / mm2.2.The IENFD and sciatic nerve function index scores of the sciatic nerve injury in the sciatic nerve injury were positively correlated at each time point(2 weeks r = 0.977,P <0.001;4 Week r = 0.956,P <0.001;6 weeks r = 0.936,P <0.001;8 weeks r = 0.996,P <0.001);rat sciatic nerve injury side of the foot IENFD and nerve conduction velocity results at all time points were positively correlated(2 weeks r = 0.860,P <0.001;4 weeks r = 0.912,P <0.001;6 weeks r = 0.728,P <0.001;8 weeks r = 0.542,P <0.001).Conclusions:Skin biopsy can be used to assess the peripheral nerve injury.IENFD is consistent with the sciatic nerve index and nerve conduction velocity during the recovery of sciatic nerve injury.