The Expression And Significance Of IL-35 Signaling Pathway Related Factors In Oral Lichen Planus

Objective:To investigate the expression and significance of IL-35signaling pathway related factors in oral lichen planus.Methods:1.40 cases of patients with oral lichen planus peripheral blood?20 cases of non-erosive type,20cases of erosive type?and 20 cases of healthy control group.The m RNA levels of Ebi3,p35,gp130,IL-12R?2,STAT1 and STAT4 in peripheral blood mononuclear cells?PBMCs?of OLP were measured by real-time fluorescence quantitative PCR?qPCR?.The above factors and OLP were analyzed.The condition and the correlation of immune indicators.2.Oral lichen planus lesions in 41 cases?21 non-erosive type,20 cases of erosive type?and 15 healthy control groups.The expression of Ebi3,p35,gp130,IL-12R?2,STAT1,STAT4 mRNA in OLP lesions was detected by real-time fluorescent quantitative PCR?qPCR?.Proteins Ebi3,p35,and gp130 in OLP lesions were detected by immunohistochemistry.The distribution and expression of IL-12R?2,and the relationship between these factors and the clinical characteristics of OLP.Results:1.The expression levels of Ebi3,p35,gp130,IL-12R?2,STAT1 and STAT4 mRNA in peripheral blood of oral lichen planus were higher than those in normal control group?P<0.05?;STAT4 was higher in erosive than non-erosive type,and the rest There was no statistical difference between the two types of OLP?P>0.05?.The correlation between the above factors and OLP immune parameters was analyzed.The results showed that the expression level of Ebi3 mRNA was related to T+B+NK cells?r_s=0.349,P=0.040?,and the expression level of STAT1 mRNA was positively correlated with IgA?r_s=0.468,P=0.003?,the expression level of STAT4mRNA was negatively correlated with Ig M?r_s=-0.334,P=0.038?.2.The expression levels of Ebi3,p35,gp130,IL-12R?2,STAT1 and STAT4 in oral lesions of oral lichen planus were higher than those in normal control group?P<0.05?;Ebi3,p35,gp130,IL-12R?2 in OLP lesion tissues.The positive expression rates ofprotein were 66%?27/41?,39%?16/41?,36.59%?15/41?,and 34.15%?14/41?,respectively,which were higher than those in the normal control group.Significance?P<0.05?;and the relative expression of Ebi3 and p35 protein was positively correlated?r_s=0.404,P=0.009?.The positive expression rate of Ebi3 protein was higher in the basal cell liquefaction group than in the low group?c²=9.172,P=0.010?;the positive expression rate of IL-12p35 protein was higher in the erosive type than in the non-erosive type?c²=7.220?,P=0.007).The positive expression rate of IL-35 protein in OLP lesions was 34%?14/41?,which was higher than that of the normal control group.The expression of IL-35 protein in erosive type was higher than that of non-erosive type?c²=4.364,P=0.037?.The difference was statistically significant?P<0.05?.The expression of gp130 and IL-12R?2 protein in erosive OLP lesions was significantly higher than that in non-erosive ones?c₁²=5.707,P₁=0.017;c₂²=4.264,P₂=0.037?,and the difference was statistically significant?P<0.05?.The relationship between IL-35 ligands and receptor expression levels was further analyzed.The results showed that IL-35 receptors and There was a positive correlation between the expression levels of ligands?r_s=0.892,P<0.001?.The positive expression rate of gp130 and IL-12R?2 protein in the OLP group had no significant differences in the course of disease,lymphocyte infiltration,and basal cell liquefaction?P>0.05?.Conclusion:1.The expression of IL-35 signaling pathway related factors in peripheral blood of oral lichen planus is increased,which is related to immunity,suggesting that IL-35signaling pathway related factors may be involved in the pathogenesis of OLP immunity.2.The expression of IL-35 and its receptors gp130 and IL-12R?2 was up-regulated in OLP lesions,which was related to the clinical classification of OLP,suggesting that IL-35 signaling pathway related factors may be involved in the formation and development of OLP lesions.