The Study Of The Quality And Decoction Of Solanum Nigrum Based On The Content Of Antineoplastic Solanine

ObjectivePharmacological study and clinical application in recent years showed that Solanum nigrum had a significant anti-tumor effect,the effective ingredient of anticancer for solanine(mainly solasonine and solamargine),in order to make better use of the medicinal value of Solanum nigrum,using high performance liquid chromatography(HPLC)of Solanum nigrum fruit herbs the solanine(mainly solasonine and solamargine)method for determination of content and content in different areas of Solanum nigrum tasn were determined on the basis of established HPLC fingerprint analysis method of Solanum nigrum and provide the basis for the identification and quality evaluation of the crude drug;in order to explore the best application of Solanum nigrum fruit(decoction)method of solasonine nigrum fruit in different liquid decoction decoction time and solamargine content were studied and the best time:decoction optimization The main drugs of Solanum nigrum Decoction best decoction.Methods(1)the solasonine from different habitats and Solanum nigrum fruit solamargine were determined by HPLC method.10 groups of different origin of Solanum nigrum by methanol reflux purification,obtained from different Solanum nigrum fruit for the test solution,using HPLC method to measure the content of solasonine and solamargine which.To compare the effects of different production on the medicinal effects of the Solanum nigrum fruit.(2)using Agilent ZORBAX SB-C18 column(150 x 4.6mm,5 m),acetonitrile-0.3% phosphoric acid as mobile phase HPLC method with gradient elution,HPLC pattern analysis of 10 groups of Solanum nigrum medicinal materials.The chromatographic fingerprint similarity evaluation system "(2004 A)for similarity evaluation,the establishment of quality standard of Solanum nigrum and plant identification to provide reliable data.(3)the solasonine Solanum nigrum fruit were measured and solamargine content by HPLC method.The different time of Solanum nigrum decoction,after concentration and purification,different decocting time nigrum fruit for the test solution,respectively measuring content of solamargine,through the determination results,ultimately determine the best decocting time from Solanum nigrum.Solanum nigrum fruit based medicine decoction and Xiaochengqi decoction combined with formula of load and.Take Xiaochengqi Decoction: Magnolia 2g,Immature Bitter Orange 2g,Radix Paeoniae Alba 2g,bupleurum 2g,rhubarb 1.6g,licorice 1.3g,adding 15 times amount of water,soaking 30 min,after boiling boiling 30 min,small Chengqi Decoction liquid.By 5g the other Solanum nigrum Decoction and Xiaochengqi Decoction decoction combined with liquid and gas decoction combined with Decoction and small bearing Solanum nigrum 5g,Solanum nigrum 5g and Xiaochengqi decoction combined with Decoction and the lack of Solanum nigrum Decoction of Solanum nigrum 4 different negative liquid medicine decocting method,the concentration and purification.4 kinds of Solanum nigrum decoction test solution.The content of solamargine by HPLC method on 4 species of Solanum nigrum Decoction were measured in the test solution.Through the comparison of the measurement results for bearing gas soup Decoction of Solanum nigrum best,achieve the effect of clinical application of optimal.Results(1)Solanum nigrum fruit alkaloids by HPLC method(mainly solasonine and solamargine)were measured,solasonine in different area of Solanum nigrum fruit and solamargine content in Jilin produces the fresh fruit of Solanum nigrum(7.0806mg/g)solasonine and solamargine(7.7705mg/g)the highest content of Solanum nigrum nuts for measuring result of Jilin,Anhui and Hebei were higher,solasonine in respectively 5.0812mg/g?4.1368mg/g?3.7721mg/g,the content of solamargine were 5.1146mg/g?4.2133mg/g?3.8497mg/g.(2)the 10 batch of Solanum nigrum medicinal fingerprints were established,among which there were 12 peaks,identified the solasonine and solamargine 2 characteristic peaks were solasonine(peak 7,retention time is 22.72min)and solamargine(peak 8,retention time is 23.41min),the chromatographic fingerprint similarity evaluation system "(2004 A)similarity of 10 batches of samples of HPLC from Solanum nigrum and control map of the evaluation indicates that the similarity is good(3)Solanum nigrum Decoction alone,30 min,20min,10 min effective content of solamargine respectively 2.37mg/ml?2.55mg/ml?2.81mg/ml;the other Solanum nigrum Decoction and Xiaochengqi decoction combined with liquids olamargine content 2.26mg/ml,Solanum nigrum and Xiaochengqi Decoction and decoction of solamargine content is 2.06mg/ml,and after the Solanum nigrum Xiaochengqi Decoction Decoction of solamargine content is 2.33mg/ml.