Identification Of Ginseng From Different Producing Areas By Non-linear Chemical Fingerprint And Determination Of Fake Content Doped In Ginseng

Identification on cultivated ginseng and quantitative evaluation on fake content doped in ginseng were investigated by non-linear chemical fingerprint, combined chromatographic technique whose advantage was analysis of the sample signal components. The non-linear chemical fingerprint has the advantage of sample cluster composition information and visible characteristics.Ginseng contained ginsenoside Re, Rgl, Rb1 and Rf by TLC. the content of ginsenoside Re, Rgl, Rbl met requirement of Chinese Pharmacopoeia by HPLC, the content of ginsenoside Re and Rgl was not less than 0.3%, the content of ginsenoside Rb1 was greater than 0.2%. Then ginseng powder samples were joined in "HT+BrO3-+Mn2++Acetone" steady chemical system, determination conditions of non-linear chemical fingerprint about ginseng were systematically studied, the optimal reaction system were sample dosage of 0.6 g, sample particle size of 100 mesh, the reaction temperature of 37℃ and the stirring speed of 850 r/min. Non-linear chemical fingerprint of ginseng from different producing areas were set up and characteristic information difference between the ginseng and some other traditional Chinese medicine (panax) were found by non-linear chemical fingerprint. System similarities of non-linear chemical fingerprint of ginseng from different producing areas were calculated using information parameter of fingerprint, the similarity between the same origin were greater than 97.12%, but less than 94.25% between different regions, a new method to identify ginseng origins by of system similarity model was put forward. The change of quantitative information after doping platycodon grandiflorum or radix glehniae was studied, and the linear regression equation between the quantitative information and falsify content was ensured by information regression method. Regression equations between oscillation life and doping platycodon grandiflorum contents in ginseng was y= 6419.4 x+ 11443, R2= 0.9914; Regression equations between induction time and doping radix glehniae contents in ginseng was y= 4478.4+3177.4 x, R2=0.9944; Whether doping adulterants (platycodon grandiflorum or radix glehniae) in ginseng and doping content were determined according to the two regression equations, thus the samples were subjected to quantitative quality evaluation. Finally, compared with chromatography and spectrum, there were summaries about characteristics and advantages of non-linear chemical fingerprint that identified and evaluated ginseng.