Influence On The Inflammatory Cytokines Of HaCaT Cells Under UVB Radiation Injury With The Treatment Of Aqueous Extract Of Lycium Ruthenicum In Qinghai Qaidam

Objective:To study the influence of inflammatory cytokines secreted from HaCa T cells under UVB radiation injury with the treatment of aqueous extract of lycium ruthenicum in Qinghai Qaidam.Method:We took the logarithmic growth phase cells as research object.The experimental group with UVB radiation injury for 12 hours and then added different concentrations(0.5、1.0、2.0mg/ml)of aqueous extract of lycium ruthenicum.The UVB model group and control group added only the same amount of culture media.After 12 hours of intervention,the cellular supernatant and precipitation were collected under 4℃,and the content of TNF-α and IL-6 of 5 groups of cellular supernatant and precipitation were measured.Results: Compared with the control group,the synthesis and secretion of TNF-α and IL-6 of HaCaT cells were siginificantly increaseed in the UVB model group(P < 0.05).While the synthesis and secretion of TNF-α and IL-6 of HaCaT cells were siginificantly reduced in the experimental group,and it presented the dose effect in a certain concentration range(P < 0.05).Conclusion: Inhibiting the synthesis and secretion of TNF-α and IL-6 may be one of the mechanism of the protection and repair of the queous extract of lycium ruthenicum in Qinghai Qaidam for the HaCaT cells under UVB radiation injury.