Residue Analysis And Digestion Dynamics Study Of 113g/L Anti-pour Ester Microemulsion On Wheat

In current work, a rapid analysis method for the simultaneous determination of trineaxapac-ethyll and trinexapac by ultra-performance liquid chromatography(UPLC-PDA) and liquid chromatography coupled with tandem mass spectrometry(LC-MS/MS) were established. In order to evaluate the residual dynamics and environmental safety of trinexapac-ethyl in wheat, studies on the terminal residue and dynamic degradation of 113 g/L trinexapac-ethyl microemulsion in soils, kernels and wheat plants were carried out respectively in Anhui, Shandong and Heilongjiang provices in 2014 and 2015. The data could help the government establish appropriate application frequency and harvest intervals in the use of trinexapac-ethyl on wheat plants.Trinexapac-ethyl belongs to cyclohexane carboxylic acid PGR, which was easily metabolized to corresponding free acid metabolite. It could supress the anaphase biosynthesis of gibberellic acid in plant, mainly through the green part of the plant absorption, the effect occured during the growth site, inhibition of cell elongation, reach the effect of lodging. Currently, few studies reported on the analysis method and residual dynamics of trinexapac-ethyl and trinexapac in wheat by UPLC-PDA and LC-MS/MS.The soil, kernel and wheat plant samples were extracted with ethyl acetate without further clean up. The residues of trinexapac-ethyl and trinexapac were determined by UPLC-PDA and LC-MS/MS.The limit of quantitation(LOQ) of trinexapac-ethyl in soil, kernel and wheat plant were 0.05, 0.05 and 0.01 mg/kg, respectively. Average recovery rates were around 77.83%-105.24% at spiked levels of 0.02-1.0 mg/kg. The LOQ of trinexapac in soil, kernel and wheat plant were 0.05, 0.05, 0.2 mg/kg, respectively. Average recovery rates ranged from 76.66% to 101.00% at spiked levels of 0.05-5.0 mg/kg. The relative standard deviations(RSDs) were <10%. The established method was sensitivity, the accuracy and precision could meet the requirements for detection of pesticide residues.The terminal residue and dynamic degradation of trinexapac-ethyl in soil, kernel and wheat plant were carried out at three fields in two years. The results demonstrated that when the applied dosage was at 110.63 mL/mu, trinexapac-ethyl and trinexapac degraded fast. Furthermore, the degradation of trinexapac-ethyl in wheat plants followed the first-order kinetics. The half-livers of trinexapac-ethyl was 3.83-7.15 days, which indicated trinexapac-ethyl belonged to the easily degraded of pesticides(t1/2<30). When the applied dosage was at 73.75 mL/mu, the terminal residue levels of trinexapac-ethyl were lower than 0.5 mg/kg in these three matrices.Moreover, the thermodynamics of degradation behavior of trinexapac-ethyl and trinexapac were also investigated. The result showed that the higher microorganism, less moisture content and stronger alkalinity of soil was the faster speed of trinexapac-ethy and trinexapac degrade.