| Purpose: To investigate the effect of sijunzitang(sjzt) decoction on the fecal bacteria,intestinal mucosal morphology, macrophage phagocytic function and IFN-? expression of digestive tract infection in mice with spleen deficiency syndrome.For the prevention and treatment of spleen deficiency syndrome of Candida infection to provides a theoretical basis.Material and method: This experiment SPF kunming mice were randoml y divided into five groups, that is normal control group(A)? spleen model group(B)?spleen+Candida albicans group(C)?spleen+Candida albicans +flucona zole group(D)?spleen+Candida albicans+sjzt group(E).The mice were fed for one week after modeling.In addition to the normal control group(A), the other four groups of mice with spleen deficiency take complex factors, continuous modeling 14 d.The 15 d,mice of group C?D?E gavage for Candida albicans(2×108CFU/m L)suspension 0.5m L/only,While giving group A?B Physiological saline 0.5 m L/only.The 16 d, group D were fed with fluconazole,group E micew erefed with sjzt.Once a day,for a week.Observe the general state of the mice in each group.In start lavage sjzt decoction 7, 14, 21 days, detectionof Candida albicans in feces.The jejunum of mice were prepared with HE staining and path ological observation of intestinal mucosal morphological changes.Determination the content of mice serum IFN-?with ELISA method.The expression level in small intestine of mice was detected by Western blotting.The level of IFN-? m RNA examined the expression of small intestineof mice by RT-PCR.Results: 1. Effect of Sjzt decoction on the general state of intestinal infection of Candida albicans in mice with spleen deficiencyCompared with the normal control group,spleen model group?spleen+ Candida albicans group the experiment of 22 day, food less poor appetite, hair withered and yellow, body weight and food intake decreased significantly, dull eyes is very narrow.The experiment of 29 day,spleen+Candida albicans group mice weight decreased significantly,the ear color pale ear after hair scattereddull,will dilute and prolapse of the anus,weak limbs swaying walk, arch back,afraid of the cold,activities reduce hi curled up together.Compared with the spleen+ Candida albicans group,spleen+Candida albicans +fluconazole group?spleen+Can dida albicans+sjzt group after administration,the general state of mice has impro ved,the 22 day of the experiment began to rise gradually,the 29 day of the Experiment,the basic shape of feces,food intake increased and so on.2. Effects of Sjzt decoction on intestinal bacteria of intestinal infection of Candida albicans in mice with spleen deficiencyCompared with the normal control group,spleen model group?spleen+Cand ida albicans group?spleen+Candida albicans+fluconazole group?spleen+Candida albicans+sjzt group the amount of fecal bacteria in mice was increased by 7? 14 and 21 days after filling the stomach with Sjzt decoction(P<0.01 or P< 0.05).Compared with the spleen model group,spleen+Candida albicans group? spleen+Candida albicans+fluconazole group?spleen+Candida albicans+sjzt group the amount of fecal bacteria in mice was increased by 7?14 and 21 days after filling the stomach with Sjzt decoction(P<0.01 or P<0.05).Compared with the spleen+Candida albicans group,spleen+Candida albicans+fluconazole group? spleen+Candida albicans+sjzt group the amount of fecal bacteria in mice was decreased by 7?14 and 21 day(P<0.01 or P<0.05).Compared with the spleen+Candida albicans+fluconazole,spleen+Candida albicans+sjzt group the amount of fecal bacteria in mice was increased 7?14 and 21 day(P<0.01 or P<0.05).3. Effects of Sjzt decoction on spleen deficiency of mice by oral Candida albicans infection in intestinal mucosal morphologyCompared with the normal control group,spleen model group?spleen+ Candida albicans group the intestinal villi of mice were sparse,the intestinal gland epithelial cell atrophy was more obvious,capillary congestion and edema. Compared with the spleen+Candida albicans group,spleen+Candida albicans+ fluconazole group?spleen+Candida albicans+sjzt group in the 7?14 and 21 days after the administration of mice intestinal villi and other structures have been restored,the intestinal glands arranged neatly,no congestion and edema.4. The effect of Sjzt decoction on the phagocytic rate of Candida albicans in the peritoneal macrophages of mice with intestinal infection after Candida albicansCompared with the normal control group,spleen model group?spleen+Candi da albicans group?spleen+Candida albicans+fluconazole group?spleen+Candida albicans+sjzt group the phagocytic rate of macrophage phagocytosis of Candida albicans in mice was decreased by 7?14 and 21 days after filling the stomach with Four Sjzt decoction(P<0.01).Compared with the spleen model group,splee n+Candida albicans group Phagocytosis of Candida albicans by mouse peritone al macrophages was decreased at 7?14 and 21 days of administration(P<0.01 or P<0.05).spleen+Candida albicans+fluconazole group Phagocytosis of Candida albicans by mouse peritoneal macrophages was increased 21 days after administ ration(P<0.05).spleen+Candida albicans+sjzt group Phagocytosis of Candida albicans by mouse peritoneal macrophages was increased 14?21 days after admi nistration(P<0.01 or P<0.05).Compared with the spleen+Candida albicans group,spleen+Candida albicans+fluconazole group?spleen+Candida albicans+sjzt group Phagocytosis of Candida albicans by mouse peritoneal macrophages was increased 7?14 and 21 days after administration(P<0.01).5. Effect of Sjzt decoction on the content of IFN-? in serum of mice with Spleen deficiency syndrome after intestinal infection with Candida albicansCompared with the normal control group,spleen+Candida albicans+fluconazo le group mice serum IFN-? on the 14 day content increased(P<0.01),Spleen+ Candida albicans+sjzt group mice serum IFN-? on the 7 and 14 day content in creased(P<0.01).Compared with spleen model group,spleen+Candida albicans+ fluconazole group the content of IFN-? in serum of mice was increased 14 day safter administration(P<0.05).spleen+Candida albicans+sjzt group the content of IFN-? in serum of mice was increased 7 and 14 days after administration(P< 0.01).Compared with the spleen+Candida albicans+fluconazole group?spleen+ Candida albicans+sjzt group the content of IFN-? in serum of mice was increas ed 14 days after administration(P<0.05).6. Effect of Sjzt decoction on the expression of IFN-? m RNA protein levels in small intestinal tissue of mice with intestinal infection after Candida albicansCompared with the normal control group,spleen model group the expression level of IFN-? in the small intestine of mice was increased by 7 and 14 days after filling the stomach with Sjzt decoction(P<0.01),spleen+Candida albicans group?spleen+Candida albicans+fluconazole group?Spleen+Candida albicans+ sjzt group the expression level of IFN-? in the small intestine of mice was incr eased by 7 ?14 and 21 days after filling the stomach with Sjzt decoction(P< 0.01).Compared with spleen model group,spleen+Candida albicans group?spleen+Candida albicans+fluconazole group?Spleen+Candida albicans+sjzt group the expression level of IFN-? in the small intestine of mice was increased by 7 ? 14 and 21 days after filling the stomach with Sjzt decoction(P < 0.01). Comp ared with the spleen+Candida albicans group,spleen+Candida albicans+fluconazol e group ?Spleen+Candida albicans+sjzt group the expression level of IFN-? in the small intestine of mice was increased 7 days after administration of the drug(P<0.01 or P<0.05).7. Effect of Sjzt decoction on the expression level of IFN-? in small intestine of mice with intestinal infection after Candida albicansCompared with the normal control group,spleen model group the expressio n level of IFN-? in small intestine of was increased at 7 and 21 days(P<0.01 or P<0.05),spleen+Candida albicans group?spleen+Candida albicans+fluconazol e group?Spleen+ Candida albicans+sjzt group the expression level of IFN-? in small intestine of was increased at 7?14 and 21 days(P < 0.01).Compared with spleen model group,spleen+Candida albicans group?spleen+Candida albica ns+fluconazole group?Spleen+Candida albicans+sjzt group the expression level of IFN-? in small intestine of was increased at 7?14 and 21 days(P<0.01 or P<0.05).Compared with the spleen+Candida albicans group?spleen+Candida albicans+fluconazole group ?Spleen+Candida albicans+sjzt group the expression level of IFN-? in small intestine tissue was increased 7 days after administratio n(P<0.01 or P<0.05).Conclusions: 1. Sjzt decoction can improve the general state of mice with spleen deficiency infection of Candida albicans,after sjzt decoction, the amount of fecal bacteria was also significantly decreased. 2. Sjzt decoction can reduce the inflammation of the intestinal mucosa of spleen deficiency mice infected with Candida albicans. 3. Sjzt decoction can improve the spleen deficiency of mice infected with Cand ida albicans of peritoneal macrophage phagocytosis ability of Candida albicans. 4. Sjzt decoction could increased the spleen deficiency mice infected with Candida albicans,the expression level of IFN-?protein and m RNA in serum of IFN-?and IFN-?in small intestine. |
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