Effects Of Different Intensity Aerobic Exercise On The Expression Of PerilipinA MRNA And GLUT-4 MRNA In Diabetic Mice

OBJECTIVE: To investigate the effects of different intensity endurance exercise on PerilipinA mRNA and GLUT-4 mRNA expression in diabetic mice.METHODS: 4 weeks old male C57 / BL6 mice were fed with high fat and high glucose for 5 weeks,then 5 consecutive days,once a day fasting streptozotocin(STZ)induced into diabetic mice model,and fasting blood glucose ? 11.1mol / L as a model to determine whether the standard mouse model.The mice were randomly divided into 8 groups: healthy and healthy control group(C,n = 10),healthy low intensity exercise group(CL,n = 10),healthy moderate intensity group(CM,n = 10)(DM,n = 10),diabetic low intensity group(DML,n = 10),diabetic moderate intensity group(DMM,n = 10),diabetic high intensity group(DMH,n = 10).After short-term adaptive training,the mice in the exercise group were trained for 7 weeks in different stages according to different intensity.During the training period,the body weight and blood glucose of each group were monitored.The expression of PerilipinA mRNA and GLUT-4 mRNA in the liver tissue of mice were determined by real-time PCR.The expression of PerilipinA and GLUT-4 mRNA were detected by western blotting.The expression of PerilipinA mRNA and GLUT-4 mRNA was detected by real-time PCR.Conclusion:1.Aerobic exercise can effectively improve the blood glucose level of diabetic mice,moderate intensity exercise effect is most significant;2.The expression of PerilipinA mRNA in diabetic mice was lower than that in control group,but only in high intensity group.3.The expression of GLUT-4 mRNA in diabetic mice was higher than that in control group,and the medium intensity was the highest.