| Objective:The purpose of study was to observe the impact of endurance and resistance movement towards the rats which suffer from diabetes and its myocardial energy metabolism cells content and related enzyme activity,and explore the influence of exercise intervention on glucolipid metabolic pathways of diastolic dysfunction with diabetes.Method: Male SD rats were randomly divided into control group(pC)and model group(pD).After 7 weeks of high fat diet and intraperitoneal injection of streptozotocin(STZ),the pD group was divided into diabetic control group(D),diabetic endurance exercise group(DA)and diabetic resistance exercise group(DR).pC group was divided into control group(C),endurance exercise control group(CA)and resistance exercise control group(CR).CA,DA,DR group and CR group were completed 8 weeks treadmill training and weight ladder training,then measured fasting blood glucose(FBG),blood lipid,insulin(FINS)and insulin resistance index(HOMA-IRI),the area under the curve of glucose tolerance test(AUC).Left ventricular catheter cardiac hemodynamic index;colorimetry myocardial glycogen,free fatty acid(FFA),lactic acid,lactate dehydrogenase(LDH),ELISA(PDH)to measure myocardial pyruvate dehydrogenase and carnitine acyl transferase(CPT-1),Western-Blot myocardial cell glucose transporter 4(GLUT4),uncoupling protein 2(UCP2).Results:1.Compared with C group,FBG,HOMA-IRI and AUC in the D group were significantly increased,body weight and FINS were significantly decreased,FBG,HOMA-IRI and AUC in DA and DR group were significantly lower than those in group D,DA and DR groups were significantly higher than those in group D.Compared with DA group,DR and AUC in group FBG were significantly lower.2.Compared with group C,serum lipid level was significantly higher in the D group,high density lipoprotein(HDL)did not change significantly;compared with group D,DA,DR group and DA group were significantly decreased,the triglyceride(TG),total cholesterol(CHOL),low density lipoprotein(LDL)than DR group obviously.3.Compared with group C,group D,left ventricular diastolic pressure(LVDP),left ventricular end diastolic pressure(LVEDP),left ventricular relaxation time constant(Tau)increased significantly,left ventricular pressure decline rate(-dp/dt max)significantly decreased;compared with D group,DA group,LVDP LVEDP,Tau decreased,-dp/dtmax increased,there was no significant difference in DA group.Compared with DA group,LVDP,LVEDP and Tau in the DR group were significantly increased,-dp/dtmax decreased significantly.4.Compared with group C,group D,LDH,PDH of myocardial glycogen decreased,free fatty acid,lactic acid and CPT-1 increased;compared with group D,group DA,LDH and PDH increased glycogen,free fatty acid,lactic acid and CPT-1 decreased significantly in group DR,glucose metabolism and enzyme substrate had no significant change,free fatty acids,the content of CPT-1 decreased significantly.5.Compared with C group,D in group GLUT4 was significantly lower,UCP2 was significantly higher than that in DA group,GLUT4 was significantly higher than that of D group,UCP2 was decreased,DR was significantly higher than D group,GLUT4 was significantly higher in group DR than group DA.Conclusion:1.Endurance or resistance exercise can improve the metabolism of glucose and lipid in diabetic myocardium,and the trend of endurance exercise is more obvious.The two kinds of exercise can also improve the blood glucose and lipid metabolism caused by diabetes,and the effect of resistance exercise on lowering blood glucose and insulin resistance is better.2.Endurance exercise can significantly improve the left ventricular diastolic function in diabetic patients,but the resistance exercise has a tendency to aggravate the left ventricular diastolic dysfunction.This may be related to the different expression of UCP2 induced by different movement. |
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