Preparation Of Core-Shell Molecular Imprinting Polymer For Lincomycin A And Its Application In Chromatographic Column

Lincomycin is a multi-component substance.In the process of production of lincomycin,there are active lincomycin A and non-active lincomycin B.Its content in the product must be strictly restricted.In this paper,it is necessary to develop new separation technology to purify lincomycin A.The separation efficiency for lincomycin A of core-shell molecular imprinting polymer.In this paper,the methyl methacrylate(MMA)were respectively used as monomer in seed core,Acrylamide(AM),methacrylic acid(MAA)and crylic acid(AA)were used as the shell functional monomers.Core-shell MIPs for lincomycin A were prepared by using lincomycin A as the template molecule,Ethylene glycol dimethacrylate(EGDMA)as the cross-linker and ammonium persulphate(APS)as the initiator.The molecular imprinting polymers of lincomycin A were prepared by core-shell emulsion polymerization.Transmission electron microscope(TEM),FT-IR spectra,energy dispersive spectrometry(EDS),thermal gravimetric analyzer(TGA)and specific surface area and pore analysis(BET)were used to characterize the molecular imprinting polymers(MIPs).The conditions of polymerization were chosen by optimizing the different monomers(AM)and the ratio of the template and functional monomer(1:10).Static adsorption and selective adsorption were used to evaluate the adsorption ability and specific recognition ability of MIPs.From the results of isothermal and dynamic adsorption,the maximal adsorption capacity can reach 62.66 mg/g,which reached equilibrium within 6 hours.The selectivity coefficients of MIPs and non-imprinted polymers(NIPs)for lincomycin A related to lincomycin B were 3.14 and 1.08.The separation of lincomycin A was detected by column chromatography.In this paper,the separation conditions were optimized by different flow rate,sampling concentration and elution.The maximal dynamic adsorption reached 15.53 mg/g.From the result,the 0.1 mol/L NaOH was used as elution,and the purity of lincomycin was 94.95%.In the end,the adsorption ability of MIPs for lincomycin A was verified in lincomycin fermentation broth.It can receive the purity of lincomycin A(93.3%)by using HCl and sodium borate.