| Molecular imprinting technique (MIT) is a polymerizing technique to produce molecular recognition materials with high selectivity and affinity. Polymerization is occurred between functional monomers and crosslinker around a template. After extration of the template from polymer, the molecularly imprinted polymer (MIP) has memory to the template. It has many applications in the fields of analytical chemistry, catalysis and organic synthesis, In normal condition, molecularly imprinted polymers only have effective recognition in non-polar or weak polar organic solutions. Strong polar solution or water could disrupt the template-monomer interaction. However, for some biological samples whose biological recognition mainly occurs in aqueous solution and the application of MIPs were limited to some extent. So it is extremely important to prepare MIPs that are able to go on biological recognition in aqueous solutions. In this paper, it was consisted of the following four parts.Chapter one: The development, principle and application of molecular imprinting technology were introduced in this paper.Chapter two: Aminoantipyrine has been employed as templates for the preparation of in-situ molecularly imprinted polymer rods for use as liquid chromatographic stationary phases. The preparation procedure was simple and easy. The recognition of the molecularly imprinted polymer rod was evaluated by comparing the retention values of Aminoantipyrine and its analogues. The results showed that Aminoantipyrine was more strongly retained than its analogues in the imprinted polymer rod-type colomn, but the blank rob with the same function monomer had no such ability. Accordingly, the mechanism of molecular recognition of this system was proposed. And the effect of chemical group and the contributions of ionic and hydrogen bond interactions on separation was also investigated.Chapter three: Molecuarly imprinted columns with high selectivity for the imprinting molecules - antipyrine, aminopyrine and aminoantipyrine in aqueous medium were prepared respectively by in situ polymerization. Both organic and aqueous mobile phases were applied for the evaluation of the chromatographic characteristics of the column. It was found that theimprinted column showed very high selectivity towards the template molecules in aqueous medium. The mechanism of molecular recognition was discussed. Ionic and hydrophobic interactions were presumed as the reasons for the molecule recognition in aqueous medium.Chapter four: Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) method was established for the determination of baicalin and berberine hydrochloride in An Gong Niu Huang Capsule. Results showed that the calibration curves were linear within the ranges of 0.08- 3.2 u g for baicalin (r=0.9991) and 0.08 - 0.8 ?g for berberine hydrochloride(r=0.9992). This method was simple, specific, accurate and reproducible.
|
PDF Full Text Request