Gypside Extracted And Separated From Chinese Traditional Medicine Gynostemma Pentaphyllum (Thunb.) Makino

In this paper,mainly study extraction and separation purification of glucoside from Gynostemma pentaphyllum(Thunb.)Makino.The total Gypside is extracted by alcohol and purified by resin,and separated through silica gel column.By orthogonal design test the optimumterms:The material liquid is to 1:12,the density of ethanol solution is 70%,it is 40 degrees Centigrade to soak temperature,it is 3 times to soak and propose the number of times.The research of separation purification of resin to the total gypside shows:resin of D-280 has better decolourization results;The loss of the gypside is 0.58mg/ml on average after decolouring.The absorbing amount of gypside is 37.47mg/mL in case of fully absorbing that the resin of AB-8 to gypside,the elution rate is 90.07%by 75%of the ethanol to elute.The optimumterms elution density is 20%,45%,75%and 90%respectively.The total gypside divide into two parts by 45%ethanol.The yield of the total gypside purified by D-280 and AB-8 is 5.09%and the content of the total gypside is 88.64%.Experiments of silica gel column to elute shows:The silica gel column is a good separation function to gypside.By experiments:Gyp2 and Gyp3 and Gyp4 are acquired.It is 42.68%that measured Gyp2 purity by HPLC,Gyp3 purity is 67.92%,Gyp4 purity is 68.29%.And separate purify to Gyp2 and Gyp3 second,the purity after the purification is that Gyp2-2 purity is 74.38%,Gyp3-2 purity is 78.72%respectively,the first silica gel column separate purity of purification raise by 31.70%and 10.80%separately.