| Botanical medicine are active ingredients with unique pharmacological and biological activities extracted from plants and play an significant role in anti-cancer,anti-tumor and other fields.The isolation and purification techniques of botanical medicine are mostly based on column chromatography such as macroporous resins and high-performance liquid chromatography(prep-HPLC).However,it must be emphasized that there are some limitations related to column chromatography such as high pressure drop,large consumption of organic solvents,and difficulty in scale-up.Membrane chromatography technology is a novel bioseparation technology which the isolation process is mainly based on the adsorption-elution mode,and the components in a sample mixture are separated according to the different interaction forces between drug molecules with different polarity and separation membranes.This separation technique effectively overcome many shortcomings of the traditional chromatographic technology and has broad application prospects in the field of botanical medicine purification.In this study,a polypropylene non-woven fabric was worked as the membrane substrate,and graphene oxide with strong drug-loading capacity was coupled to the non-woven fabric surface to obtain a polypropylene hydrophobic chromatographic film.A model drug pomegranate peel polyphenols was purified by hydrophobic interaction and ?-? interactions between GO and diverse drug molecules using polypropylene hydrophobic chromatographic membrane.The details are as follows:(1)Polypropylene hydrophobic chromatographic membranes were prepared by photografting maleic anhydride onto polypropylene,aminolation,coupling with graphene oxide.Using FT-IR,UV,Raman,SEM and other means to analysis chromatographic membran,and explore its adsorption capacity.Research indicates the GO grafting degrees on the polypropylene membrane was 0.37wt.%,The adsorption capacity of the model drug cinnamic aldehyde was 1.45 mg/g.(2)This study created a method for estimating the relative hydrophobicity of a drug,and the elution time corresponding to membrane chromatographic purification of the drug was estimated based on the relative hydrophobicity.The solubility parameters and relative retention time(tRR)of avariety of model drugs were measured based on their molecular structure.And the mathematical relationship between the solubility parameter and tRR was established(tRR =-0.032+7.27144e-?t/3.53874,R=0.97).(3)The model drug pomegranate peel polyphenols was purified by hydrophobic polypropylene chromatographic membranes.The effects of adsorption phase pH,temperature and injection concentration on the reversible adsorption capacity of polypropylene hydrophobic chromatographic membranes were investigated.The research found a suitable conditions for the dynamic adsorption of polyphenols with the adsorption phase of pH 2 HCl aqueous solution and the membrane module temperature of 30?.The higher the sample concentration,the stronger the adsorption capacity of the chromatographic membrane for polyphenols.(4)The purity and recovery of the purification products were determined by HPLC,Folin-Ciocalteu and the like.Under a suitable separation condition(adsorption phase and elution phase is pH 2 HC1 aqueous solution and 0.06M NaOH aqueous solution respectively,membrane module temperature is 30?,injection concentration is 1.5 mg/ml),99.5%of polysaccharide and 95.3%of the protein could be removed after a purification,the purity of the polyphenols increased to 89.3%,and 36.9%of polyphenols could be recovered.(5)Proper operating conditions for membrane modules were studied.The pressure drop increased to 27 kpa when the flow rate is 3 ml/min,which is much lower than the column chromatographic pressure at the same flow rate(pressure was about 22.76Mpa);After 9 of adsorption-elution cycles in the same conditions using polypropylene hydrophobic chromatography membrane,there was no obvious change of the reversible adsorption capacity on polyphenol,and the membrane was reusable. |
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