Research On Anti-radical Activities Of Glycyrrhetinic Acid Derivatives And Industrial Design Of Licorice Products

Glycyrrhetinic acid as one of the active ingredient of Licorice,which is more representative significance of anti-aging medicine active ingredients,has a good application prospect some specific fields,such as food,cosmetics,and daily chemical and so on.In this paper,we used mono ammonium glycyrrhizinate as raw material for the preparation of glycyrrhetinic acid,and used the resulting glycyrrhetinic acid for preparing its metal complexes.Then use electrochemical method was used for researching the anti-free radical activities of glycyrrhetinic acid and its metal complexes,and total flavonoids of licorice.In addition,we also designed the batch production preliminary process of licorice products.Specific as follows:1.Mono ammonium glycyrrhizinate which was prepared by laboratory,as raw material for preparing glycyrrhetinic acid and iron?Fe?,copper?Cu?,zinc?Zn?,calcium?Ca?metal complexes.Glycyrrhetinic acid and its complexes were analysed by UV-visible absorption spectrum and infrared absorption spectrum,to identify the four complexes.The initially speculating structure of glycyrrhetinic acid complexes,shows that the coordination bond occurs in the position of 11 carbonyl group and the30 carboxyl group.2.We investigated the ability for scavenging free radicals of glycyrrhetinic acid and its four kinds of metal complexes,b y using the changes of peak current in the electrochemical analysis method for comparison parameters.The results show that:glycyrrhetinic acid and its four kinds of complexes all have effect on scavenging free radicals.The free radical scavenging effects for DPPH exhibit that:Glycyrrhetinic acid-Zn>Glycyrrhetinic acid-Ca>Glycyrrhetinic acid-Fe>Glycyrrhetinic acid-Cu>Glycyrrhetinic acid,compare to Glycyrrhetinic acid,it increases 34.2%,32.2%,28.13%,21.23%,respectively.The coordination of Zn²⁺and Ca²⁺with Glycyrrhetinic acid have greatly increase the ability of scavenging DPPH;The free radical scavenging effects for Hydroxyl free radical?·OH?exhibit that:Glycyrrhetinic acid-Zn>Glycyrrhetinic acid-Cu>Glycyrrhetinic acid-Fe>Glycyrrhetinic acid-Ca>Glycyrrhetinic acid,compare to Glycyrrhetinic acid,it increases 19.19%,19.15%,8.68%,0.89%,respectively.The coordination of Zn²⁺and Cu²⁺with Glycyrrhetinic acid have greatly increase the ability of scavenging Hydroxyl free radical;The free radical scavenging effects for Superoxide radical?O₂·^-?exhibit that:Glycyrrhetinic acid-Zn>Glycyrrhetinic acid-Cu>Glycyrrhetinic acid-Ca>Glycyrrhetinic acid-Fe>Glycyrrhetinic acid,compare to Glycyrrhetinic acid,it increases 30.98%,21.84%,11.0%,3.41%,respectively.The coordination of Zn²⁺and Cu²⁺with Glycyrrhetinic acid have greatly increase the ability of scavenging Superoxide radical;The free radical scavenging effects for Lipid peroxyl radical?ROOH?exhibit that:Glycyrrhetinic acid-Fe>Glycyrrhetinic acid-Ca>Glycyrrhetinic acid-Ca>Glycyrrhetinic acid-Zn>Glycyrrhetinic acid-Cu>Glycyrrhetinic acid,compare to Glycyrrhetinic acid,it increases 24.47%,12.58%,4.35%,2.53%,respectively.The coordination of Fe³⁺and Ca²⁺with Glycyrrhetinic acid have greatly increase the ability of scavenging Lipid peroxyl radical.3.By screening the optimum conditions of modifying agents,buffers,buffer p H,scan rate,we established an electrochemical method to detect the total flavonoids of licorice waste residue,and used this method to detect the scavenging effect of total flavonoids of licorice waste residue on free radicals.The scavenging results show that:DPPH?66.3%?>lipid peroxyl radical?ROOH??55.97%?>Hydroxyl free radical?·OH??44.44%?>Superoxide radical?O₂·^-??25.64%?.It is shown that licorice flavonoids have a strong scavenging effect on DPPH and lipid peroxide radical.4.We designed the preliminary process for monoammoniun glycrrhizinate,total flavonoids of licorice,liquorice residue,and drew the process flow diagram.