The Study Of Zanthoxylum Bungeanum Leaves Functional Oral Solution

The leaves of Zanthoxylum bungeanum has very high nutritional and medicinal value,which are of great potential application in nutraceutical and functional food products for improving human health.Plant drinks are famous in international and domestic academics for their nutrition and safety.In this study,flavonoid antioxidants from Z.Bungeanum leaves were extracted and purified as the major ingredient for preparing the oral solution.The sterilization method of the oral solution was also investigated,as well as its stability.The results are as follows:(1)Ultrasonic-assisted extraction was a better choice comparing with microwave-assisted extraction and immersing extraction,the index of this method were optimized by response surface: the leaves were dried at ambient temperature in the dark,and were finely ground in a laboratory grinder.The powder was sieved by a sieve with the particle size of 40 meshes and then was soaked by 25 times 60% ethanol solution,an 400 w,50? ultrasonic treatment was performed for 15 min twice to get the crude flavonoids extracts.The content of quercitrin,hyperoside,rutin and afzelin of the the extracts were 29.87,42.73,38.74 and 5.00 mg/g,respectively.The content of total flavonoid and total phenolic were 120.35 ?mol equiv.QUE/100 g and 89.77 ?mol equiv.GAE/100 g,respectively.The FRAP value and ABTS value were 409.92 and 744.05 ?mol Trolox/g,respectively.The DPPH IC50 value was 18.09 ?g/ml.(2)The optimal ATPS conditions were chosen as 29%(w/w)Na H2PO4,25%(w/w)ethanol concentration,1%(w/w)added amount of leaves extracts,no p H adjustment,1h extraction at 25 ? for twice.Under the optimal conditions,The content of quercitrin,hyperoside,rutin and afzelin of the the extracts were 44.78,65.55,56.42 and 6.84 mg/g,respectively.The content of total flavonoid and total phenolic were 153.26 ?mol equiv.QUE/100 g and 112.93 ?mol equiv.GAE/100 g,respectively.The FRAP value and ABTS value were 619.22 and 965.52 ?mol Trolox/g,respectively.The DPPH IC50 value was 10.47 ?g/ml.The optimal D-101 macroporous resin adsorption parameters were initial concentrations in sample solution of 7.5 mg/m L,p H of 5.0,sample loading amount of 5 BV,flow rate of 2 BV/h.The optimal desorption parameters were deionized water and 30% ethanol each 5 BV,then 70% ethanol of 10 BV,flow rate of 2 BV/h.after purified,the content of quercitrin,hyperoside,rutin and afzelin of the the extracts were 76.52,104.09,94.45 and 9.75 mg/g,respectively.The content of total flavonoid and total phenolic were 210.32 ?mol equiv.QUE/100 g and 161.61 ?mol equiv.GAE/100 g,respectively.The FRAP value and ABTS value were 975.38 and 1595.37 ?mol Trolox/g,respectively.The DPPH IC50 value was 7.31 ?g/ml.So macroporous resin purification was choosen as the better enrichment method.(3)The formula of the oral solution was 3% extracts,8% xylitol,0.03% aspartame,0.015% menthol,dissolved in deionized water,store at 4 ? for 12 h.The supernate of the mixture was prepared by centrifugation at 4000 rpm for 15 min,then 0.05% potassium sorbate was add.The oral solution was packaged 10 ml/bottle.The content of quercitrin,hyperoside,rutin and afzelin of the the oral solution were 2.17,2.77,2.57 and 0.213mg/ml,resectively.The content of total flavonoid and total phenolic were 60.09 ?mol equiv.QUE/ml and 47.44 ?mol equiv.GAE/ml,respectively.The FRAP value and ABTS value were 29.22 and 45.52 ?mol Trolox/ml,respectively.The DPPH IC50 value was 32.47 ?g/ml.(4)The oral solution was sterilized by high-pressure steam sterilization method(121?,15min).The content of quercitrin,hyperoside,rutin and afzelin of the the oral solution were 1.97,2.34,2.22 and 0.199 mg/ml,resectively.The content of total flavonoid and total phenolic were 58.32 ?mol equiv.QUE/ml and 41.99 ?mol equiv.GAE/ml,respectively.The FRAP value and ABTS value were 27.38 and 41.72 ?mol Trolox/ml,respectively.The DPPH IC50 value was 34.17 ?g/ml.(5)Stability test showed that the oral solution has fine stability.According to the predictive test,the oral solution could be stored at 25±2 ?for at least 12 month.And this prediction has been validated with the storge test for 6 monthes(data was obtained for only 6 monthes so far).