Potential HIV Virus Reactivation Constituents Research From Polygonum Cuspidatum Sieb.et Zucc.

As one of the most fatal diseases of human,HIV/AIDS is still lack of ways to be cured completely in clinic at present.New method,named "shock and kill",was developed and through the potential HIV virus reactivation activity evaluation model,the fraction eluted with 30%ethanol from the macroporous adsorption resin column subjected by 60%ethanol extract of rhizome Polygonum cuspidatum Sieb.et Zucc.was discovered that it has obvious reactivity for potential HIV virus.We use macroporous resin,silica gel column chromatography,HW-40C resin gel column chromatography,ODS column chromatography,HPLC and other chromatographic methods connected with activity screening to insure the active sections and we got some active fractions.From the active fractions we isolated and identified one active compound.Tannins from the active fraction were characted using MALDI-TOF MS combined with NMR analysis for the first time.Epicatechin/Epigallocatechin was the basic units occurring in condensed tannins of active fraction.The degree of polymerization of condensed tannins from 3 to 14 was observed totally.The structure of compound one was elucidated by means of spectra analyses,and it was separated from Polygonum cuspidatum Sieb.et Zucc.for the first time.For further evluating the activity of the active fractions and compound by the model of the potential HIV virus reactive mode-assay,we found that fractions and compound both could reactive the potential HIV virus with obvious concentration-dependent effect.Also we found that active sections could make the postive control drug prostratin more effective which indicate that they have a collaborative function.