Study On Extraction And Decolorization Of Sapindus Suponins

Sapindus is a deciduous tree and mainly distributes in the eastern China,southwest and the the south of Huaihe River.Sapindus saponins is a triterpenoid saponin having an oleanolin-based saponin,which is one of the main active ingredients of the sapindus fruit.It is a natural non-ionic surfactant,ancient known as handwahing.Sapinus fruit with antibacterial,anti-inflammatory and other biological activity,is widely used in the field of biomedicine.Because it has excellent foaming performance and strong decontamination capacity,can be used as natural cosmetics raw materials.With the economic development,people pay more attention to the development of natural environmental protection products.At present,our natural product extraction and separation research has made a breakthrough.Since sapindus saponins is present in the form of a complex mixture,it is one of the difficult problems in its purification,content determination,and structural analysis.At the same time,due to the limitations of the existing technology,the application of sapindus saponins is still in the theoretical research stage.This has led to a more waste of resources without industrial applications.Currently the industry had used traditional processes such as water extraction,alcohol extraction,etc.to extract sapindus saponins.However,there are still existing problems with these processes,such as flavonoids,proteins,sugar,polyphenols and other impurities are extracted,leading to its separation and purification becomes difficult.While the traditional extraction process affects the purity and color of the product,making it very low added value.Therefore a greener and more economical production process needs to be developed,which is aimed at the low purity and other practical problems in the traditional extraction process.High purity sapindus saponins can be used as a standard for quality control.In this thesis,sapindus saponins were prepared by using macroporous resin and foam separation,meantime its content were measured by UV-Vis.The chemical components of sapindus saponins are analyzed by UV-Vis?FT-IR?HPLC and MS.(1)The results demonstrate that,the adsorption rate and desorption rate of D101 macroporous resin were the highest among the six resins(AB-8,D101,ADS-21,S-8,H103,NKA-?)under static test;When the resin column was dynamically adsorbed and separated,the saturated adsorption capacity of D101 macroporous resin was 481.57 mg and the purity was 31.51%.Therefore,it was concluded that the D101 macroporous resin had good separation effect on sapindus saponins;(2)The best conditions for the purification of sapindus saponins by foam separation were as follows:weighed its powder after separation of the resin and prepared 100 mL solution with 0.03 mg mL-1 until no bubble was generated.Three times the feed,and control the gas speed of 4 min L-1.The best separation performance,yield of sapindus saponins for quality control of 19.68%,enrichment ratio of 1.96 and purity of 92.92%,is obtained at the foam separation;(3)In this thesis,a rapid and simple method for the determination of sapindus saponins was established.We selected 0.3 mL of 5%vanillin-glacial acetic acid and 0.7 mL perchloric acid,heated at 70 ? for 15 min,ice-water bath for 3 min and then add 5 mL of glacial acetic acid,Shake,measured at 480 nm.This method has the advantages of stability and good reproducibility,and the detection result is accurate,the operation is convenient and quick,which provides the basis for the quality control of the sapindus saponins;(4)The sapindus saponins for quality control were prepared with two-step preparation by macroporous resin and foam separation.The components of it were mainly triterpenoid saponins systematically investigated by characterization techniques such as FT-IR,HPLC and HPLC-MS.The components were as follows:Sapindosides B(molecular 882),Mukurozi-saponin El(molecular 924)and Mukurozi-saponin G(molecular 966);(5)In view of the current problem of sapindus saponins decolorization,the two methods including macroporous resin and of H2O2 were investigated.The results show that the product has high purity and light color after decolorization of macroporous resin.The process is complicated and costly,and because of the high loss rate of sapindus saponins isn't suitable for industrialized large-scale decolorization.H2O2 is an ideal bleaching agent.Sapindus saponins solution by adding low concentrations of H2O2 can have a good decolorization effect.The best conditions for decolorization process were as follows:add 5 mL 30%H2O2 at 70 ?for 1.0 h,color of product is light yellow after decolorization.UV-Vis and IR characterization showed that H2O2 had a very good decolorizing effect and the sapindoside structure was not destroyed.Besides,the surface activity is basically not affected after decolorization.Therefore,H2O2 method is suitable for industrial large-scale decolorization.