Study Of Antioxidant Effects Of Three Kinds Of Drinking Water

Objective To know the water quality characteristics and evaluate the antioxidant capacity both in vitro and in vivo of three different kinds of drinking water,giving people some references for choosing which kind of drinking water.Methods Water quality parameters are detected during the trial period,including p H value,total dissolved solids(TDS),oxidationreduction potential(OR P),electrical conductivity(EC),and contents of calcium,magnesium,zinc,copper,manganese,selenium,nitrate and sulfate radical are determined too.The effects on antioxidant capacity of three different kinds of drinking water were studied in vitro and in vivo.The assessment of the antioxidant capacities of three kinds of drinking water in vitro were conducted by the free radicals scavenging capability of 2,2-diphenyl-1picrylhydrazy(DPPH),2,2-azinobis-3-et-hylb-enzthiazoline-6-sulphonate(ABTS),hydroxyl radical and superoxide anion radical.The assessment in vivo were studied in drosophila melanogasters and ICR mice.Three groups of mediums were made by three kinds of drinking water as souce of water separately.1-day-old drosophilas were fed up in three groups of mediums for 14 days.There were 10 drosophila vials in each group and 20 drosophilas were collected in each vials.After the trial period,the content of malondialdehyde(MDA)and activities of superoxide dismutase(TSO D),catalase(CAT),peroxidase(POD),glutathione peroxidase(GSH-Px),glutathione Stransferase(GST)in drosophilas were determined.These part of experiment w ere studied in ultraviolet exposure too.Sixty ICR mice(half male and half female)were randomly divided into three groups and fed with pure water,tap water and filtered tapwater respectively for 90 days.After the trial period,the total antioxidant capacity,the inhibiting ability of hydroxyl radical,content of MDAand activities of TSOD,CAT,POD,GSH-Px,GST inserums,livers and kidneys of the mice were determined according to the correponding kits methods.Observing fluorescence of liver and kidney tissues by frozen section technique.Results 1.The contents of minerals in tap water and filtered tapwater were higher from a general view.Calcium,magnesium and zinc were richest in filtered tapwater while copper and manganese were richest in tap water.The p H value of filtered tapwater was(7.71±0.02)and ORP value was(-211.00±23.00)m V,and the scavenging capabilitie of filtered tapwater was higher than others(P<0.05)[pure water(-11.22±3.78)U/m L,tap water(-16.81±2.88)U/m L,filtered tapwater(48.33±2.28)U/m L].2.Antioxidant indices in drosophilas fed up in normal condition had no diference in three groups.In ultraviolet exposure feeding condition,the contents of MDA in pure water group(1.92±0.31)nmol/mg prowas higher than tap water group(0.93±0.08)nmol/mg pro(P<0.05),and the activity of GST in tap water group(463.32±52.59)U/mg pro was higher than that in pure water group(273.41±48.13)U/mg pro and filtered tapwater(291.86±49.27)U/mg pro.3.Antioxidant indices in mice serums had no diference in three groups.In liver tissues,the TAOC of the mice in tap water group(4.69±0.49)U/mg pro was higher than that in pure water group(3.06±0.30)U/mg pro,and the activity of GSH-Px in filtered tapwater group(filtered tapwater group 344.40±14.12)U/mg pro was higher than that in pure water group(261.72±21.59)U/mg pro,meanwhile the activity of GST in tap water group(1047.56±56.18)U/mg pro was higher than that in pure water group(776.18±64.97)U/mg pro(P<0.05).In kidney tissues,the activity of TSO D in tap water group(310.24±7.95)U/mg pro was higher than that in pure water group(274.98±7.80)U/mg pro,and the activityof GSH-Px in filtered tapwater group(308.54±13.29)U/mg pro was higher than that in pure water group(252.67±11.30)U/mg pro(P<0.05).4.The fluorescence of liver and kidney tissues in pure water group is stronger than others.Conclusion Three kinds of drinking water could have different antioxidan t effects.Filtered tapwater could have stronger antioxidant capacity in vivo.Filtered tapwater and tap water may be more helpful for improving the acti vities of antioxidant enzymes and reducing the damage of oxidative stress.