Study On The Composition Of Wild Cyclocarya Paliurus

In the paper,the response surface method was used to optimize the extraction process of the total sugar and total flavonoids from C.paliurus leaves in Guizhou Leishan area,and the chemical components were extractd,separated and analysed.This experiment collected C.paliurus leaves in 11 regions of the country,and the fingerprint was studied by high performance liquid chromatography?HPLC?.1.Box-Behnken method was used to optimize the ultrasonic extraction process of cellulase from total sugar and flavonoids of C.paliurus leaves.Based on four single factors including solvent type,ratio of material to liquid,ultrasonic time and amount of cellulase,the optimum process was optimized by four factors and three levels,and the content of total sugar and flavonoids in C.paliurus leaves in different months was determined by combining the standard curve with the best technological conditions,and setting up the curve of its change.The optimum conditions for total sugar and flavonoids were as follows:?1?Total sugar:1:8.38 g/mL of water extract,ultrasonic time 27.3 min,cellulase dosage 0.2%.?2?Total flavonoids:1:44.5 g/mL of 75%ethanol,ultrasonic time 29 min,cellulase dosage 0.454%.Under these conditions,the extraction rate of polysaccharides was 3.746%,and the extraction rate of flavonoids was 5.472%.Compared with the total sugar and total flavonoids in different months,the polysaccharide content was the highest in April and lowest in June,and the total flavonoids content was the highest in August and lowest in April.By comparing the content of total sugar and total flavonoids in different regions,it could be seen that the content of polysaccharides and flavonoids in C.paliurus leaves in Jiushijiu area was generally higher than that in Dunan area.Compared with the content of total sugar and total flavonoids in different provenances,the content of polysaccharides in C.paliurus leaves of seed seeding was generally higher than those in wild plants,and the content of flavonoids in wild plants was generally higher than those in seed seeding.2.The components of petroleum ether extract from C.paliurus leaves were analyzed through GC-MS,and 44 compounds were acquired.The 44 substances were classified into 6 categories including aliphatic,aromatic,aromatic,alkaloid,steroid and terpenoid compounds.3.The influence of wavelength,mobile phase and extraction site on chromatogram was investigated by HPLC.The optimum chromatographic conditions were as follows:the detection sample was 50%methanol extract.The chromatographic column was DIONEX C₁₈?250×4.6 mm?.And the mobile phase was as follows:acetonitrile-0.1%phosphoric acid solution,gradient elution,0⁸ min,2%¹0%A;8³9 min,10%²5%A;39⁵7 min,25%³2%A;57⁷0 min,32%⁶0%A;70⁸5 min;60%⁹0%A;85⁹0 min,90%⁹5%A.The volume flow rate was 1:1.0 mL/min.The column temperature was 35?.The sample volume was10?L.The detection wavelength was 254 nm.Under this condition,The HPLC was used to detect the C.paliurus leaves from11 producing areas.The National Pharmacopoeia Commission's"similarity Evaluation system of chromatographic fingerprint of traditional Chinese Medicine"evaluated the fingerprint of 11 batches of Chinese medicinal materials from different producing areas and different companies in China.It was found that there were 20peaks in C.paliurus leaves from 11 producing areas.The cluster analysis divided them into 5 categories.Class?included S1?Hunan?,S2?Zhejiang?,S11?Jiangxi?,S5?Guizhou?.Class?included S3?Guangxi?,S9?Hubei?,S6?Guizhou?.Class?included S4?Jiangxi?,S10?Guizhou?.Class?included S7?Henan?.Class V included S8?Zhejiang?.The principal component analysis showed that peak 14,peak18,and peak 8 were characteristic peaks of C.paliurus leaves in 11 different places.