Synthesis And DNA-binding Properties Of Complexes On Benzothiazole Derivatives

Benzothiazole complexes are widely distributed in nature,which are widely used in industry,agriculture,medicine and other fields because of its good biological activity.Four benzothiazole ligands were synthesized in this paper.Zn?II?,Ni?II?and Co?II?compounds?1-3?based on 2-?2-pyridyl?benzothiazole?bpt?and terephthalic acid?PTA?were synthesized.The crystal structures of[Zn?bpt??PTA?₂]?1?,[Ni?bpt??PTA?₂]?2?,and[Co?bpt??PTA?2]?3?have been determined by single-crystal X-ray diffraction analysis,which shows that all the three complexes belong to monoclinic system with space group P2₁/C.Time-dependent density functional theory?TD-DFT?calculation is performed on a reference structure of complex 3.The excited electrons mainly localized at the?*of ligand 2-?2-pyridyl?benzothiazole,which will be convenient for them to bind with the DNA reacting sites.XRD and TG were performed on three complexes to characterize the properties.Fluorescence spectroscopy,ultraviolet?UV?spectroscopy,CD and viscosity were used to characterize the interaction of the complexes with Calf thymus DNA?CT-DNA?.The results indicate that complexes 1-3 bind to CT-DNA via a non-intercalative mode.This mode can effectively break DNA and prevent DNA replication.we hope to provide a theoretical basis for the design and development of new anti-cancer drugs.