Investigate The Effect Of PHGG On Mice Model Of Depression Based On Intestinal Flora

With the increasing pressure of competition in modern society,a growing number of people are suffering from depression;however,drugs for treating depression have serious adverse effects.In recent years,related studies have shown that the intestinal microflora were associated with nerve-related diseases,thus modulating the metabolites,especially short chain fatty acids(SCFA),of intestinal flora by some dietary supplements may be of positive significance to treat or relieve nerve-related diseases.Previous research suggests that partially hydrolyzed guar gum(PHGG)can alter the abundance of intestinal microflora,with increased the levels of lactic acid,acetic acid,propionic acid,and valeric acid,which may potentially be helpful for the relief of depression.Therefore,this study established a depression mice model to investigate whether PHGG alleviates the neurobehavior of depression by modulating intestinal microflora.The main content of this research is as follows:1.The mice depression model was established by incorporating of chronic unpredictable mild stress(CUMS)method with single cage feeding.The eligible model mice determined by behavioral assessment methods,subsequently were administrated PHGG by means of intragastric administration to evaluate the effects of PHGG on depression in mice.2.The level of lactic acid and SCFA including acetic acid,propionic acid,butyric acid and valeric acid in the intestine of mice were determined by high performance liquid chromatography(HPLC)to investigate the effect of PHGG on intestinal metabolites in depressed mice.3.Determination of neurotransmitters in mice by HPLC to investigate the effect of PHGG on depression.4.On the basis of this work,the effect of PHGG on human intestinal metabolites was further explored through in vitro fermentation experiments.Results:1.The results of behavioral assessment show that the prevention and intervention of PHGG changed the depressive behavior of mice with increased percentage of the sugar-water preference,reduction of immobility time for forced swimming,increased in-field time in open-field experiments,and increased time of horizontal and vertical in open-field experiments compared with the depressed mice,indicating that PHGG hads a certain benefit effects on relieving depression.2.HPLC tests of lactic acid and SCFA in the intestinal metabolites of PHGG-administrated mice showed a moderate increase,compared with the depressed mice.3.The results of neurotransmitter tests show that the levels of neurotransmitters DA and 5-HT in the blood,hippocampus and striatum of PHGG-administrated mice were higher than those in depressed mice,demonstrating that PHGG relieve depression in mice.This revealed that PHGG modulated the intestinal flora,increased the levels of acetic acid,propionic acid,valeric acid,and elevated the levels of neurotransmitters,thereby improving the effect of relieving depression.4.The results in vitro fermentation products showed that the concentration of lactic acid,acetic acid,propionic acid,butyric acid and valeric acid increased after fermentation of human feces treated by PHGG,in comparison to the control group.The findings indicated that PHGG had a noteworthy regulation effect on the metabolites of human intestinal flora,suggesting that PHGG may achieve prevention and improvement effect on depression in human by regulating the intestinal flora.