The Hypolipidemic Effect Of Camellia Seed Meal Polysaccharide And Its Influence On Intestinal Flora

Camellia seed meal is the main by-product after oil extraction of Camellia seed,with high polysaccharide content.At present,the research on the polysaccharides from Camellia oleifera seed meal is mainly limited to improve extraction rate,explore antioxidant and hypolipidemic activities.However,the influence of the intestinal flora,the effect of lowering blood lipids and the mechanism of action of the polysaccharides from camellia seed meal are still unclear.A large number of studies have suggested that dyslipidemia can lead to intestinal flora disorder.Therefore,this paper uses camellia seed meal as raw material to extract and purify the polysaccharide components of camellia seed meal,and conduct preliminary structural characterization to study its effect on the intestinal flora of normal mice and its effect on lipid metabolism in hyperlipidemia mice.And use the intestinal flora as an entry point to explore the potential mechanism of action.The main results are as follows:(1)Two polysaccharide components of Camellia oleifera seed meal were extracted and purified and analyzed for their structureThe crude polysaccharide of Camellia seed meal(CCP)was obtained by hot water extraction and ethanol precipitation,and the two salt-washed polysaccharide components,CCPA and CCPB,were separated and purified by DEAE-Cellulose DE-52.The two components of polysaccharides have the basic structure absorption peaks of polysaccharides,both contain uronic acid and furanose rings,and may be acid polysaccharides.The uronic acid content of CCPA and CCPB were 10.27%and 5.43%,respectively.The molecular weight of CCPA is 7.02×10~4Da,and the content of galacturonic acid is relatively high;the molecular weight of CCPB is 2.46×10~6Da,and the content of arabinose is relatively high.Through basic structure analysis,it is speculated that the inherent viscosity of CCPA is less than that of CCPB.(2)Camellia seed meal polysaccharides can improve the intestinal function and flora structure of normal miceCompared with the control group of mice,after the intervention of CCPA and CCPB polysaccharide components,the body weight of the mice did not change significantly,but the food intake of the mice was significantly increased.At the same time,the weight of the cecum,the digestion and absorption of carbohydrates and short-chain fatty acid content rose remarkably,while gastrointestinal transit time,p H and ammonia content in feces reduced significantly.High-throughput sequencing showed that CCPA and CCPB can up regulate the number of probiotics and down regulate the number of pathogenic bacteria,and CCPA has a more obvious effect.(3)Camellia seed meal polysaccharides can improve lipid metabolism,intestinal function and flora structure in hyperlipidemia miceCompared with the blank group of mice,after treatment with CCPA and CCPB polysaccharide components,the body weight,epididymal fat index,serum TC,TG,LDL-C content and liver TC and TG content,fat accumulation of high-fat mice The colon index,the thickness of the muscularis and mucosa of the colon,the HDL-C content in the serum and the water content in the intestinal tract increased significantly.Qualcomm sequencing found that after the intervention of CCPA and CCPB,the structure and diversity of gut microbes in high-fat mice changed.The abundance of beneficial bacteria in the mouse intestine increased while the abundance of pathogenic bacteria decreased,indicating that the two-component polysaccharides can help to restore the balance proportion of intestinal flora in high-fat mice.