Preparation Of Antibacterial And Hemostatic Multifunctional Materials And Its Structure And Properties

Uncontrolled bleeding is the main cause of death in clinical and military trauma,the wounds become a hotbed of bacteria to cause bacterial infection,so bleeding and wound infection are associated,they are threat to human life and health.At present,the construction of antibacterial hemostatic materials is an important measure to deal with hemorrhagic injuries and related infections.Hemostatic sponge is one of the important hemostatic materials.It is an important research direction to develop the antibacterial properties of hemostatic sponge without changing its hemostatic properties.Tissue adhesive refers to the glue or patch that binds tissues together to stop bleeding by directly or indirectly causing the formation of blood clots.It is a kind of hemostatic material supplemented by hemostatic sponge material.It makes up for the limitation of the application range of hemostatic sponge and is of great significance for hemostasis.Therefore,this paper adopts the strategy of combining hemostasis control and anti-infection to conduct the preparation and performance research of two types of antibacterial hemostatic materials.?1?A surface-adaptive,on-demand antibacterial hemostatic sodium alginate/gelatin sponge is fabricated by sequential spray assisted layer-by-layer assembly of the fungicide of poly?hexamethylene biguanide?hydrochloride?PHMB?and the biological compatibility of hyaluronic acid?HA?for the first time,which overcome the defects of toxic effects to the human body after the use of PHMB.The hemostatic sponge with negatively charged hyaluronic acid can prevent the exposure of fungicide PHMB,so the coated sponge has good biocompatibility.Once bacteria invade,the secreted hyaluronidase could degrade the upper HA layer under physiological conditions,and then the exposed PHMB layer could kill the bacteria.The coated sponge shows excellent biocompatibility,as negatively charged HA can prevent the exposure of bactericidal PHMB.Moreover,the coated sponge exhibited excellent hemostatic properties in vitro and in vivo by aggregating and activating blood cells.Our strategy provides a novel approach to the design of surface adaptive and on-demand bactericidal coatings on the surface of hemostatic sponges,which has the advantage of avoiding infection complication during hemostasis.?2?Tissue adhesive refers to the glue or patch that binds tissues together and stops bleeding by directly or indirectly causing the formation of blood clots.It is of great significance for hemostasis.In this paper,the hydrogen bond between tannic acid?TA?with catechol structure and polyethylene glycol?PEG?with excellent biocompatibility was utilized,and the antibacterial biological tissue adhesive(TA/CNCs-PEG₂₀₀₀/VAN)with good adhesion strength and high mechanical properties was formed by natural biological crystalline cellulose nanocrystals?CNCs?.Studies had shown that the content of polyethylene glycol chain accounts for about 56%of CNCs-PEG₂₀₀₀,and TA/CNCs-PEG₂₀₀₀/VAN is formed through the hydrogen bond between TA and PEG,and TA/TA/CNCs-PEG₂₀₀₀/VAN had the function of releasing sterilization,which can prevent wound infection.In vitro physiological conditions simulating in vivo physiological conditions were designed to demonstrate the gradual degradation of TA/CNCs-PEG₂₀₀₀ over time.Our strategy provides a new direction for the development of biological tissue adhesives,which can avoid the infection during hemostasis and expand the application range of hemostatic materials.