Preparation Of CS/PVA Electrospun Nanofibrous Membranes And Its Mechanical,Antibacterial And Slow-release Properties

Nanofiber membranehas been widely used in wound dressing,drug delivery and other fields because of its excellent properties such ashigh porosity,good permeability and good adsorption.Electrospinning is widely used to prepare nanofiber membranes because of its advantages of simple process and low cost.The nanofiber membrane prepared from chitosan(CS)has good biocompatibility,antibacterial and antibacterial properties,so ithas been widely prepared and studied.However,there are still many limitations of chitosan nanofiber membrane,such as poor mechanical strength,poor absorption,limited antibacterial performance,etc.Herein,the shortcomings of chitosan/polyvinyl alcohol nanofiber membrane are improved.Throughhot-melt treatment,carrying chitosan oligosaccharide(CHOS),allicin(Alli),graphene oxide(GO)and other fillers,preparation can be used for wound dressing materials and other fields,with antibacterial slow-release CS/PVA nanofiber membrane.In this work,chitosan(CS)and polyvinyl alcohol(PVA)were used as the main raw materials to prepare nanofiber membrane by electrospinning.In order to improve the strength of nanofiber membrane,the post-treatment of nanofiber membrane was carried out by means ofhot melting.The effects of temperature on the properties of nanofiber membranes were studied.The effects ofhot-melt treatment on the morphology of nanofiber membranes were studied by SEM.The changes of mechanical properties before and afterhot-melt treatment were tested by mechanical experimental machine.It was found that the mechanical properties of the nanofiber membrane afterhot-melt treatment were significantly improved,especially after 100? treatment,the effect was the best,and the tensile strength was increased by more than 89%.In order to solve the problem of insufficient absorption of CS/PVA nanofiber membrane due to the large molecular weight of CS,CS/PVA nanofiber membrane loaded with small molecular weight chitosan oligosaccharide(CHOS)was spun in this paper.The properties of CS/PVA/CHOS nanofiber membrane were investigated by means of electron microscopy,bacteriostatic test and water contact angle test.The result of SEM shows that CS/PVA/CHOS nanofiber membranehas a good fiber forming effect.The antibacterial test shows that the antibacterial activity of the nanofiber membrane loaded with 10wt%CHOS is increased by about 38%,and the water contact angle test shows that the nanofiber membranehas goodhydrophilicity,which shows that the addition of CHOS effectively improves the defects of CS based nanofiber membrane,such as difficult molecular release and general antibacterial effect.In view of the problem that the bacteriostasis of CS/PVA nanofiber membrane is not outstanding enough,Alli was selected as the carrier in this paper,and CS/PVA/Alli nanofiber membrane was successfully spun.The bacteriostasis and cytocompatibility of the nanofiber membrane were investigated by bacteriostatic test and cytotoxic test.The results showed that the antibacterial performance of nanofiber membrane was improved by 90%after the loading of 2wt%Alli.however,the drug release test showed that CS/PVA/Alli nanofiber membranehad poor drug release effect,so GO was introduced as the carrier to adjust the release rate of Alli,and CS/PVA/Alli/GO nanofiber membrane was prepared.The results of the release test showed that 0.1%GO could effectively slow down the release rate of Alli and make the drug nanofiber membranehave the effect of slow-release.Herein,CS/PVA nanofiber membranes with good antibacterial,slow-release andhydrophilic properties were prepared by means ofhot-melt modification of CS/PVA nanofiber membranes and carrying different fillers,which improved the application potential of CS/PVA based nanofiber membranes in wound dressing,tissue engineering and other biomedical fields.