Taraxacum Mongolicum Root And Its Synergistic Hypoglycemic Effect With Pueraria Root And Astragalus

Today,chronic diseases have seriously endangered human health,and diabetes is one of them.Therefore,medicinal and food homologous plants with strong biological activity and high safety have attracted much attention and can be used as functional foods and drugs to lower blood sugar to improve type 2 diabetes.The Taraxacum Mongolicum root was used as the raw material in this study.In order to study the active function of extracts from Taraxacum Mongolicum root by different solvents.The antioxidant effects of different solvent extracts were compared.The effects of different extracts on the hypoglycemic ability were studied.And analysis of active ingredients in different extracts.The best solvent extract of Taraxacum Mongolicum root was selected and mixed in Pueraria root and Astragalus membranaceus,and the ability to lower blood glucose was explored.Through insulin-induced HepG2 cell experiments,verify the ability to reduce glucose of each extract and compound.Final preparation of hypoglycemic granules.Provide theoretical basis for the development and utilization of Taraxacum Mongolicum root.Effect of Solvent Polarity on Activity of Taraxacum Mongolicum roots Extract.Extraction with 6 solvents of different polarities.Results indicated that solvent polarity had a substantial influence on the extraction efficiency from Taraxacum Mongolicum root leaves and the antioxidant activities of the extracts.As the polarity of the solvent decreases,the extraction rate and antioxidant activity also decrease.The water extract had the highest crude extract yield of 24.87%.Water extract also revealed the best DPPH racial scavenging capacity,the best hydroxyl radicals scavenging capacity,the best ABTS+·scavenging capacity,and the best reducing power.With the increase of the concentration,the six extracts'inhibitory rate of ?-glucosidase and ?-amylase also increased.When the concentration reached 5 mg/mL,the inhibition ability of ?-glucosidase and ?-amylase was the strongest under water extraction conditions,reaching 71.56%and 74.9%.Correlation analysis of Taraxacum Mongolicum root extract composition and function.The highest content of polysaccharides in the six solvent extracts was water extraction,the content was 63.92 mg/g;The highest content of total flavon and total phenols was ethanol extract,the content was 10.03 mg/g and 12.26 mg/g;The highest content of methanol extract saponin is 0.88 mg/g.According to the correlation analysis between the active ingredients in the extract and the anti-oxidant of the hypoglycemic agent,it can be known that polysaccharides are the main components that affect biological activity.Synergistic effect of Taraxacum Mongolicum root water extract and pueraria and astragalus on hypoglycemic ability.Taking the inhibitory ability of ?-glucosidase as an indicator,the optimal synergistic ratio of Taraxacum Mongolicum root,pueraria radiata,and astragalus root was determined.By establishing an in vitro insulin resistance HepG2 model,the effects of different proportions of compound components on glucose metabolism were determined.The results showed that insulin resistance was improved in each group,and the effects of the compound group on the glucose metabolism were significantly different compared with the single group.In the best synergistic group,when Taraxacum Mongolicum root:Pueraria root:Astragalus is 1:1:3,it has the highest glucose consumption and liver glycogen content,and has the strongest effect on HK and PK activity.Study on Preparation of Taraxacum Mongolicum,Pueraria Root and Astragalus Granules.The experimental results show that the ratio of extract powder:mannitol:microcrystalline cellulose is 3:2:1.Forming rate reached 91.94%.When the amount of aspartame is 0.5%,the taste is optimal.When picking up ethanol with a concentration of 80%as a binder,the effect of preparing particles is best.The quality of the particles was evaluated.The angle above the angle of repose was 35.3°,and the relative humidity was 69%,which met the requirements for particle preparation.It provides a new way for the development and application of Taraxacum Mongolicum.At the same time,it provides a theoretical basis for the development of hypoglycemic drugs and functional foods,and has guiding significance for application.