Preparation And Biological Characteristics Of Peony Seed Oil Microcapsules

Peony seed oil is an emerging oil with high application value.It is rich in unsaturated fatty acids(> 90%)and its content of ?-linolenic acid is as high as 45%,which has attracted widespread attention.However,peony seed oil is also susceptible to the external environment and rancidity occurs,which limits the application of peony seed oil in the market.Therefore,it is inevitable to develop a multi-functional and applicable peony seed oil product.In the study,peony seed oil was used as the research material to prepare the peony seed oil microcapsule emulsion and its microcapsules,and the simulated digestion process in vitro and biological characteristics of the peony seed oil emulsion in vivo were investigated.The effects of properties and antioxidants on the oxidative stability of peony seed oil microcapsules were investigated.The main results are as follows:(1)The dynamic viscosity,emulsification stability and particle size of the emulsion as the detection indicators,the effect of different wall material combinations,emulsifier combinations and oil content on the emulsion was investigated.The best emulsion preparation process was obtained: 70% of water phase,30% of solid content,wall material selects sodium starch octenyl succinate(SSOS)/ ?-cyclodextrin(?-CD)/ pectin(70%: 25%: 5%),polyglycerin fatty acid ester / lecithin(1:1,2% of solid content)as emulsifier,and the core material accounts for 35% of solid content.The stability of the emulsion prepared under this process condition was more than 99%,and the particle size was only 5.86 ?m.(2)The effect of pectin concentration on the physical and chemical properties of peony seed oil microcapsules were explored.The rheological properties of peony seed oil emulsion and the physical and chemical properties of peony seed oil microcapsules after spray drying were investigated.The results showed that the ratio of SSOS,?-CD and pectin was 70%: 22.5%: 7.5%(w/w),the particle size of the microcapsule emulsion was 4.52 ?m,the highest embedding rate was 92.50%,the lowest surface oil content was 2.60%,and the moisture content was 1.76%.The observation of tungsten filament scanning electron microscope showed that the prepared peony seed oil microcapsule particles were spherical,with smooth surface and no obvious wrinkle.Simultaneous thermogravimetric analysis showed that compared with unencapsulated peony seed oil,the peony seed oil microcapsules had good thermal stability.(3)The effects of different pectin contents on simulated digestion of peony seed oil microcapsule emulsion in vitro and the effects of different peony seed oil emulsion dosages on the biological characteristics of animals were systematically investigated.During the process of simulated digestion in vitro,the fatty acid release rate was the fastest and the fatty acid release rate reached 80.00% without adding pectin,and in the simulated gastric juice digestion process,droplet aggregation occurred and the particle size of emulsion in gastric digestion increased to 8.67?m.In the combination with pectin added,the combination of 70% SSOS / 22.5% ?-CD / 7.5% pectin can play a good slow-release effect on free fatty acids in peony seed oil,and the FFA release rate was 82.50%.In vivo experiments,different dosages of peony seed oil microcapsules had no significant effect on the body weight of rats.Compared with the HF-G group,the HDL level of the HP-G group increased significantly(p <0.05).At the same time,the peony seed oil microcapsule emulsion had a good effect on the inhibition of TC and TG levels in SD rats.Among liver indicators,peony seed oil microcapsules had good inhibitory effects on both ALT and AST transaminase,indicating that peony seed oil microcapsule emulsion had good blood lipid lowering function.(4)Peony seed oil microcapsules containing three different natural antioxidants(tea polyphenols,?-tocopherol,ascorbic acid)were prepared by spray drying technology.The results of oven accelerated experiments showed that the three natural antioxidants could enhance the storage stability of the peony seed oil microcapsules through the detection of the POV and TBARs values of the microcapsules,Among them,tea polyphenols had the strongest antioxidant activity.After accelerated storage for 4 weeks,the POV value was 78.30 meq/kg,which effectively inhibited the oxidation of peony seed oil powder oil.According to the antioxidant activity,the order was tea polyphenols> ascorbic acid> ?-tocopherol.The effects of different tea polyphenol doses on the antioxidant activity of peony seed oil powder oil were investigated,and it was found that the antioxidant activity increased with the increase of the tea polyphenol dose,indicating that there was a certain quantitative effect relationship between the antioxidant activity of peony seed oil microcapsules and the tea polyphenol dose.