The Efficacy And Safety Of Itrafungol Oral Solution On The Treatment Of Dermatophytosis In Cats

With the rapid development of economy,China's pet industry is booming.Due to the increasing number of people raising pets and pet skin disease incidence rate of diagnosis increased,the demand for skin care products is also growing.Thus,It is very necessary to develop new drug types or new drug dosage forms with lower toxic side effects.Itraconazole is a broad spectrum of antifungal drugs,against dermatophytes has significant inhibition and killing effect.In human medicine as oral agents have been used in clinic,but in the field of veterinary medicine is no development.In recent years,Itraconazole in veterinary clinical application to feline tinea infection treatment and clinical use in the security and safety assessment is imminent.The purpose of this test is to study the antibacterial activity of Itraconazole to Microsporum canis which is the feline tinea infection mainly pathogenic bacteria in vitro and all trails was conducted in the body of "the pet with an antibacterial drug efficacy evaluation test guiding principle" and "pet used antimicrobial drug field efficacy test finger under the guiding principles".To an objective and scientific evaluation the anti-sensitivity of Microsporum canis of Itraconazole Oral Liquid,the micro broth dilution method was used in this study.And all study was conduct as the M38-A2,"the sporulation of filamentous fungi broth dilution antifungal susceptibility test reference scheme" is proposed in the National Clinical Laboratory Standards Committee NCCLS/CLSI in 1998.For Microsporum canis of antimicrobial susceptibility test,through naked eye observation bacteria liquid turbidity method to determine the target drugs to the strains of the corresponding Mic(minimum inhibitory concentration).The results showed that Itraconazole was 0.25 g/mL on quality control strain of Candida albicans ATCC 10231 MIC,consistent with the reported literature.Itraconazole against clinical isolates of Microsporum canis mic for 0.125-0.5 ?g/mL,the sensitivity of Itraconazole and reported in the literature of clinical isolated Microsporum canis basically.The results showed that Itraconazole was excellent for Microsporum sensitivity on pet veterinary clinic.Phase II clinical trial of Itraconazole oral solution and the period of the northern area of clinical trials testing according to the provisions of the "pet with anti microbial drug efficacy evaluation test guidelines and" pet with anti microbial drugs were evaluated in field test guiding principle ",the feline tinea infection of cats from set up a total of five test groups,,negative control group(blank control),positive control group(drug)and the recommended dose for half dose group,recommended dose group,recommend doubling dose group.The recommended dose group and drug control group,each 30,the other group of 10.For Itraconazole Oral Solution Itrafungol control drugs(Eli Lilly&Company Limited,Elanco Animal Health).Animal experiment all Zhengzhou a stray dogs and cats rescue station selected body weight quite,varieties of gender not limited to 90 adult cats,in addition to no other diseases feline tinea bacteria infection in clinic,blood routine and blood glucose testing no abnormalities,selected in line with the requirements.Test through on animal subjects clinical signs(body temperature,respiration,heart rate),clinical disease index(with or without dander,itching,broken hair hair loss,bleeding,ulceration),blood routine,examination of clinical biochemistry,Microsporum canis negative rate of relevant indicators,and has carried on the statistical analysis to the related indicators.It was found that Itraconazole oral solution in accordance with 5 mg/kg administered,Sid(once a day),Po,7 days of continuous use,interval of 7 days,such as a course of treatment,continuous use 3 courses,with significant curative effect for feline tinea bacteria disease,and imported drugs Itrafungol®(the main component of Itraconazole are quite effective.The ill treatment of cat safety evaluation showed,Itraconazole oral solution in 1?2 times the recommended dose use using of clinical cat is safe.Itraconazole Oral Liquid three phase region of the south of clinical trials in accordance with the provisions of the "pet with anti microbial drugs were evaluated in field test guiding principle",of 60 cases meet the test requirements of the feline tinea infection and other diseases affected cats(n=30)were oral Itraconazole drug treatment effect experiment.Laboratory animals are the animal hospital of Huazhong Agricultural University,Wuhan,a pet hospital,Wuhan,a stray dog station pick body weight is quite,the variety of sex is not limited to 60 adult cats.Research methods ibid.The results showed that the drug of feline tinea bacteria infection has significant curative effect,and with imported drug Itrafungol®(main component for Itraconazole),the effect of pathogen clearance rate decreased by 90%.The ill treatment of cat safety evaluation showed,Itraconazole oral liquid had no adverse effects vesus with the control group,indicating that the drugs on the cat in clinical use is feasible.