Effects Of Summer-autumn Tea Powder On Intestinal Microecology And Lipid Metabolism Of Piglets

In this study,weaning piglets were selected as the research object.Seventy-two piglets(Duroc × Large White × Landrace)with about 11.5±0.20 kg weight was selected and randomly divided into 4 treatments,3 replicates in each group each treatment with 3 group and and 6 piglets each.At the age of 31 days,the control group was fed basal diet,and tea powder in each experimental group were added 1%,2% and 3% in the basic diet;6 replicates per group.The trial was to assess the effect of different levels of Summer-Autumn Tea Power supplementation(1%,2%,3%)on growth performance,fat metabolism,and intestinal flora structure and antioxidant performance of piglets.the summer-autumn tea powder could affect the body fat metabolism by changing the intestinal flora.The results as follows: 1.Effects of summer-autumn tea powder supplementation in piglets’ diets on intestinal microecology in pigletsSummer-autumn tea powder could change the structure of gut microbiota.Supplementation 1% and 2% summer-autumn tea powder could significantly increase the number of cecum total bacteria and Lactobacilli,the number of cecum and ileum Bacteroidetes and Bifidobacterial was increased,E.coli in cecum and ileum was significantly decreased than the control group(P<0.05).The Bacteroidetes/Total bacteria ratio were significantly higher,while Firmicutes /Total bacteria ratio were significantly lower than the control group(P<0.05).The Firmicutes/Bacteroidetes ratio was also significantly lower than the control group.(P<0.05).Compared with the control group,the number of bacteria in the duodenum and the proportion of total bacteria were not significantly different.Summer-autumn tea powder could change the number and proportion of intestinal bacteria,at the same time,influence the small intestine villi structure.The small intestine villi height increased and the villi height /depth of crypts ratio decreased than the control group.Indicating that summer-autumn tea powder could improve the intestinal microecology of piglets.2.Effects of summer-autumn tea powder supplementation in piglets’ diets on fat metabolism in pigletsIn the weaning piglets diet to add a certain concentration level of summer-autumn tea powder,not only affect the fat metabolism related to blood biochemical indicators,but also affect the content of adipocytokines.Compared with the control group,supplementation 1% summer-autumn tea powder could significantly decrease the TG content of liver and the VLDL content of liver and muscle.Supplementation 2% summer-autumn tea powder could significantly decrease the CH content of liver,the CH and TG content of muscle and FFA content of serum(P<0.05).Supplementation 3% summer-autumn tea powder could significantly increase the CH content of serum(P<0.05).In treatment group,the INS content of plasma was significantly decreased(P<0.05)Supplementation 1% summer-autumn tea powder could significantly increase the ADP content in plasma of piglets(P<0.05).The LEP,RES,TNF-α content of plasma in the test group were no significantly(P>0.05).In treatment group,the INS and RES content of liver were significantly decreased(P<0.05);The LEP,ADP,TNF-α content of liver in the test group were no significantly(P>0.05).Compared with the control group,the RES content of muscle was significantly increased(P<0.05);The ADP content of muscle was significantly increased when fed a basal diet added with 1% summer-autumn tea powder(P<0.05).Supplementation1% and 2% summer-autumn tea powder could significantly decrease the TNF-α content of muscle(P<0.05).The INS,LEP content of muscle in the test group were no significantly(P>0.05).3.Effects of summer-autumn tea powder supplementation in piglets’ diets on growth performance and antioxidant activity of pigletsThere was no significant difference between the treatment group and the control group.But the backfat thickness of each test group was significantly reduced(P<0.05),The muscle fat content increased significantly when fed a basal diet added with 2% summer-autumn tea powder(P<0.05).The SOD content of liver and muscle were significantly increased,and the MDA content of liver was significantly decreased compared with the control group(P<0.05).Compared with the control group,supplementation 1% and 2% summer-autumn tea powder could significantly increase the T-AOC,GSH-Px content of liver and CAT,GSH-Px content of muscle.Compared with the control group,supplementation 2% summer-autumn tea powder could significantly increase the CAT,GSH-Px content of serum and T-AOC content of muscle.It is suggested that the addition of summer-autumn tea powder could improve antioxidant performance of piglets.The serum biochemical indexes showed that,the summer-autumn tea powder had no significant effect on the TP,ALP,AST and BUN content of serum in the piglets.It was proved that diets supplemented with summer-autumn tea powder had no effect on the liver and kidney function of piglets.Summer-autumn tea powder is safe for piglets as feed.To sum up: supplement 1%~3% summer-autumn tea powder in piglets feed could improve the intestinal microecology;and to a certain extent,by affecting the content of adipocytokines to regulate the growth of fat metabolism of pigs.A large number of studies have shown that,the Bacteroidetes and Firmicutes are related to fat metabolism.Therefore,we believe that summer-autumn tea powder can affect the intestinal flora structure through the regulation of fat metabolism.At the same time,supplementation the summer-autumn tea powder has no effect on pig growth performance,significantly reducing its backfat thickness.And it could enhance the antioxidant capacity of piglets,is a healthy natural feed resources.1%~2% summer-powder tea power is more appropriate as pig feed.