Analysis Of The Evolution And Abiotic Stress Response Of Tomato CIPK Genes

Tomato(Solanum lycopersicum)is grown worldwide due to the various uses and high nutritional value of its fruit,but sometimes drop the production because of the adverse environment.Low temperature?drought and hight salt are major abiotic stresses,seriously affecting plant growth.Abiotic stress causes a brief and rapid increase in calcium concentration in plant cells,starting with the calcium signaling system under abiotic stress.CIPK plays an important role in plant stress response and growth and development as an important protein in calcium-mediated signal transduction pathway.However,the CIPK gene family of tomato and its response to abiotic stress have not been reported yet.The main findings are as follows:(1)In this study,22 SlCIPK gene family members were identified by bioinformatics methods,including intron enrichment and intron deletion,which can be divided into eight subfamilies according to their species.There are multiple cis-acting elements in the 1500 bp sequence upstream of SlCIPKs that respond to different stresses and plant hormones.Predicting the interaction between SlCIPKs and SlCBLs.Prediction of protein interactions suggests that one SlCBL protein interacts with one or more SlCIPK protein.Similarly,one SlCIPK protein can also interact with one or more SlCBLs.The expression of SlCIPKs in different transcripts of the tomato transformants at 0h,1h and 12 h at low temperature(4°C)(2)Using qRT-PCR to detect the expression patterns under abiotic stress(low temperature,high salt,drought),the results showed that all the members of SlCIPK family had different responses to abiotic stress,of which the changes of SlCIPK1 and SlCIPK8 were obvious,Real-time PCR showed that the expression of SlCIPK1 increased significantly during cold stress,and the expression of SlCIPK8 significantly increased under the three treatments at different time points,indicating that SlCIPK1 and SlCIPK8 may be involved in the response of different abiotic stresses.(3)Silencing the gene SlCIPK1 and SlCIPK8 in tomato seedlings by virus-induced gene silencing,respectively,followed by silencing the expression of ascorbate peroxidase,catalase,superoxide dismutase in hypoxia,drought and salt stress Proline and malondialdehyde.The results showed that the cold resistance of SlCIPK1 and SlCIPK8 knock-out plants was significantly lower than that of the normal plants,while the plants of SlCIPK1 and SlCIPK8 knock-out plants had less drought resistance,It was demonstrated that SlCIPK1 and SlCIPK8 may be associated with cold and drought resistant response.(4)In order to further study the low temperature signaling pathway mediated by SlCIPK1 and SlCIPK8,a high quality low temperature tomato yeast two-hybrid cDNA library was successfully constructed and identified.Two bait vectors were successfully constructed: pGBKT7-SlCIPK1,pGBKT7-SlCIPK8,used for screening Kam cryogenic temperature and SlCIPK1,SlCIPK8 interaction protein.Preliminary screening of a protein interacting with SlCIPK1.This study lays the foundation for the functional study of tomato CIPK gene family,and has some knowledge about its response under abiotic stress and the signal transduction pathway.