Functional Analysis Of SLMYBl4 Gene Of Tomato In Response To Abiotic Stress

Because of good taste,wide variety,rich nutrition,high research value and economic value of tomato,it has become the most widely cultivated vegetable crop in China and even in the world.At the biological level,tomatoes are also used for a variety of disciplines,a very important model plant for genetic evolution research.However,adversity stress factors in the natural environment affect the cultivation and production of vegetable seriously.Therefore,there are more crucial meaning to cultivating stress-resistant varieties of vegetable,understanding the mechanism of plant resistance to stress and exploring stress-resistant gene resources.MYB family of transcription factors,is one of the largest gene families in plants,it plays a more important role in plant growth and development,stress resistance,anthocyanin synthesis and hormone regulation.Currently,the function of MYB family of transcription factors has been studied in many crops,and some studies have shown that some genes of the MYB family in tomato were overexpressed for abiotic stress,this shows the importance of MYB transcription factors for stress resistance of plant.However,there are still many genes in the tomato MYB family that have not been found to function againt abiotic stress.Therefore,in this experiment,VIGS gene silencing and bioinformatics analysis were used for identifing and screening out some of the genes related to stress resistance in the MYB family and verifing their functions.The aim is to explore the resources of stress resistance genes and lav a theoretical foundation for the molecular breeding of stress-resistant varieties of tomato and other plants.(1)Sioinformatics analysis was used in this experiment,127 MYB transcription factors of tomato were identified.Combined with previous studies on the resistance of MYB transcription factor family members,we select 27 candidate genes from different subfamilies in the MYB family.Combining the technology of and evolutionary tree construction and chromosome mapping,these transcription factors were divided to 10 sub-families,which were been distributed in 10 of the 12 chromosomes of tomato,and the structure of most transcription factors were conservative.(2)By partitioning to the phylogenetic tree of 27 tomato MYB candidate genes,a total of 10 MYB transcription factors of tomato were picked out in each partition,and tissue specific analysis for 10 tomato MYB transcription factors were performed.The results show,the relative expression abundance of SLMYB6,SLMYB8 and SLMYB14 genes were higher levels in different tissues;the relative expression abundance of SLMYB2,SLMYB3,SLMYB7 and SLMYB10 genes were higher in root;the relative expression abundance of SLMYB1 gene was higher in buds;the relative expression abundance of SLMYB11 gene was higher in leaves.(3)The expression of 10 tomato MYB transcription factors were analyzed in abiotic stress.The results show,in drought stress,there was generally an upward trend of the relative expression abundance of the two genes of SLMYB10 and SLMYB14;In salt stress,there was no obvious trend of the expression for most genes,the relative expression abundance of 10 genes were higher at 6h and 9h basically;In low temperature stress,there was an upward trend of the relative expression abundance of SLMYB7 gene.(4)VIGS technology was used to analyze the function of SLMYB14 gene,the results showed that drought resistance and salt tolerance of tomato were reduced after silencing the SLMYB14 gene.